Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. vi-vi(1)

Transmembrane-Sequence-Dependent Overexpression and Secretion of Glycoproteins in Saccharomyces cerevisiae
pp. 1-7(7)
Authors: Schuster M.; Wasserbauer E.; Aversa G.; Jungbauer A.

Affinity Purification of Csk Protein Tyrosine Kinase Based on Its Catalytic Requirement for Divalent Metal Cations
pp. 8-12(5)
Authors: Sun G.; Budde R.J.A.

Extensive N-Glycosylation Reduces the Thermal Stability of a Recombinant Alkalophilic Bacillus -Amylase Produced in Pichia pastoris
pp. 13-23(11)
Authors: Tull D.; Gottschalk T.E.; Svendsen I.; Kramhøft B.; Phillipson B.A.; Bisgård-Frantzen H.; Olsen O.; Svensson B.

-Galactosidase of Penicillium chrysogenum: Production, Purification, and Characterization of the Enzyme
pp. 24-29(6)
Authors: Nagy Z.; Kiss T.; Szentirmai A.; Biró S.

Individual Expression of Recombinant - and -Tubulin from Haemonchus contortus: Polymerization and Drug Effects
pp. 30-39(10)
Authors: Oxberry M.E.; Geary T.G.; Winterrowd C.A.; Prichard R.K.

Expression and Purification of Recombinant Giardia Fibrillarin and Its Interaction with Small Nuclear RNAs
pp. 40-48(9)
Authors: Ghosh S.; Ghosh R.; Das P.; Chattopadhyay D.

Overexpression of Human Cardiac Troponin in Escherichia coli: Its Purification and Characterization
pp. 49-59(11)
Authors: Lohmann K.; Westerdorf B.; Maytum R.; Geeves M.A.; Jaquet K.

Expression and Purification of Extracellular Penicillin G Acylase in Bacillus subtilis
pp. 60-64(5)
Authors: Yang S.; Huang H.; Zhang R.; Huang X.; Li S.; Yuan Z.

Cloning and Expression in Pichia pastoris of Metalloprotease Domain of ADAM 9 Catalytically Active against Fibronectin
pp. 65-70(6)
Authors: Schwettmann L.; Tschesche H.

Effect of Copy Number on the Expression Levels of Hepatitis B Surface Antigen in the Methylotrophic Yeast Pichia pastoris
pp. 71-80(10)
Authors: Vassileva A.; Arora Chugh D.; Swaminathan S.; Khanna N.

Purification and Characterization of Hexahistidine-Tagged Cyclohexanone Monooxygenase Expressed in Saccharomyces cerevisiae and Escherichia coli
pp. 81-86(6)
Authors: Cheesman M.J.; Kneller M.B.; Kelly E.J.; Thompson S.J.; Yeung C.K.; Eaton D.L.; Rettie A.E.

Expression and Purification of Cytokine Receptor Homology Domain of Human Granulocyte-Colony-Stimulating Factor Receptor Fusion Protein in Escherichia coli
pp. 87-91(5)
Authors: Tatsuda D.; Arimura H.; Tokunaga H.; Ishibashi M.; Arakawa T.; Tokunaga M.

Human pS2/Trefoil Factor 1: Production and Characterization in Pichia pastoris
pp. 92-98(7)
Authors: Kannan R.; Tomasetto C.; Staub A.; Bossenmeyer-Pourié C.; Thim L.; Nielsen P.F.; Rio M-C.

Expression and Purification of the DNA-Binding Domain of the Human Transcription Factor E2F1
pp. 99-104(6)
Authors: Zeng W.; Zhang X.; Tu X.; Guang S.; Xiao Y.; Shi Y.

Expression of the Aspergillus aculeatus Endo- -1,4-mannanase Encoding Gene (man1) in Saccharomyces cerevisiae and Characterization of the Recombinant Enzyme
pp. 105-114(10)
Authors: Setati M.E.; Ademark P.; van Zyl W.H.; Hahn-Hägerdal B.; Stålbrand H.

Bacterial Expression of an Immunologically Reactive PCV2 ORF2 Fusion Protein
pp. 115-120(6)
Authors: Liu Q.; Willson P.; Attoh-Poku S.; Babiuk L.A.

Production of Functional Antibodies Generated in a Nonlytic Insect Cell Expression System
pp. 121-128(8)
Authors: Li E.; Brown S.L.; Dolman C.S.; Brown G.B.; Nemerow G.R.

Purification and in Vitro Folding of Recombinant Human Thrombopoietin Receptor Expressed in Escherichia coli
pp. 129-133(5)
Authors: Ng C.K.F.; Huxtable S.; Xu P.; Hsieh D.P.H.

Refolding and Purification of Bothropstoxin-I, a Lys49–Phospholipase A₂ Homologue, Expressed as Inclusion Bodies in Escherichia coli
pp. 134-140(7)
Authors: Ward R.J.; de Oliveira A.H.C.; Bortoleto R.K.; Rosa J.C.; Faça V.M.; Greene L.J.

Purification and Kinetic Characterization of CTP:Phosphocholine Cytidylyltransferase from Saccharomyces cerevisiae
pp. 141-148(8)
Authors: Friesen J.A.; Seo Park Y.; Kent C.

Separation of Native and Latent Forms of Human Antithrombin by Hydrophobic Interaction High-Performance Liquid Chromatography
pp. 149-155(7)
Authors: Karlsson G.; Winge S.

Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronectin in Pichia pastoris
pp. 156-164(9)
Authors: Marty C.; Scheidegger P.; Ballmer-Hofer K.; Klemenz R.; Schwendener R.A.

One-Step Separation from Lactose: Recovery and Purification of Major Cheese-Whey Proteins by Hydroxyapatite—A Flexible Procedure Suitable for Small- and Medium-Scale Preparations
pp. 165-169(5)
Authors: Rossano R.; D'Elia A.; Riccio P.

Two-Step Purification of d(-)-Specific Carbamoylase from Agrobacterium tumefaciens AM 10
pp. 170-175(6)
Authors: Sareen D.; Sharma R.; Nandanwar H.S.; Vohra R.M.

Recombinant Human Insulin VIII. Isolation of Fusion Protein–S-Sulfonate, Biotechnological Precursor of Human Insulin, from the Biomass of Transformed Escherichia coli Cells
pp. 176-182(7)
Authors: Tikhonov R.V.; Pechenov S.E.; Belacheu I.A.; Yakimov S.A.; Klyushnichenko V.E.; Boldireva E.F.; Korobko V.G.; Tunes H.; Thiemann J.E.; Vilela L.; Wulfson A.N.

Cloning, Expression, and Purification of Histidine-Tagged preS Domains of Hepatitis B Virus
pp. 183-191(9)
Authors: Núñez E.; Wei X.; Delgado C.; Rodríguez-Crespo I.; Yélamos B.; Gómez-Gutiérrez J.; Peterson D.L.; Gavilanes F.

High-Level Expression of Human Acidic Fibroblast Growth Factor and Basic Fibroblast Growth Factor in Silkworm (Bombyx mori L.) Using Recombinant Baculovirus
pp. 192-200(9)
Authors: Wu X.; Kamei K.; Sato H.; Sato S-i.; Takano R.; Ichida M.; Mori H.; Hara S.

One-Step Purification of Recombinant Yeast 6-Phosphofructo-2-kinase after the Identification of Contaminants by MALDI-TOF MS
pp. 201-209(9)
Authors: Dihazi H.; Kessler R.; Eschrich K.

High-Yield Expression, Purification, and Characterization of the Recombinant Diisopropylfluorophosphatase from Loligo vulgaris
pp. 210-219(10)
Authors: Hartleib J.; Rüterjans H.

Rapid Evaluation and Optimization of Recombinant Protein Production Using GFP Tagging
pp. 220-223(4)
Authors: Rücker E.; Schneider G.; Steinhäuser K.; Löwer R.; Hauber J.; Stauber R.H.

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
pp. 224-234(11)
Author: Tan S.

Bacterial Expression, Purification, and Characterization of Rat Hydroxysteroid Sulfotransferase STa
pp. 235-242(8)
Authors: Sheng J.J.; Duffel M.W.

High Yield Expression and Single Step Purification of Human Thionein/Metallothionein
pp. 243-250(8)
Authors: Hong S-H.; Toyama M.; Maret W.; Murooka Y.