Prothymosin
Is a Nonspecific Facilitator of Nuclear Processes: Studies of Run-on Transcription
Authors: Trumbore M.W.; Berger S.L.
Source: Protein Expression and Purification, Volume 20, Number 3, December 2000 , pp. 414-420(7)
Publisher: Academic Press
Abstract:
The effect of prothymosin
on transcriptional elongation has been examined. The addition of prothymosin
to COS-1 and NIH3T3 cell nuclei engaged in run-on transcription stimulated RNA synthesis approximately two- to threefold in a dose-dependent manner. Polyglutamic acid or a random polypeptide composed of glutamic acid, alanine, and tyrosine, did not substitute for prothymosin
. Enhanced transcription occurred in the presence of high and low doses of actinomycin D and in the presence of
-amanitin, but not in nuclear extracts. The stimulatory effect was dependent on a limiting concentration of one nucleoside triphosphate and was nearly abrogated by saturating levels of precursors. In the presence of Sarkosyl, which itself increases transcription, prothymosin
was almost ineffectual. The data are consistent with a model in which prothymosin
does not interact directly with polymerases but, instead, nonspecifically decreases the barriers to diffusion of charged molecules in electrostatically charged environments.
Language: English
Document Type: Research article
Affiliations: Section on Genes and Gene Products, National Institutes of Health, Building 8, Room 311A, Bethesda, Maryland, 20892-0480
Publication date: 2000-12-01
- In this: publication
- By this: publisher
- In this Subject: Anatomy & Physiology
- By this author: Trumbore M.W. ; Berger S.L.

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