Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. iv-iv(0)

A Nuclear Transforming Factor That Converts the Goat Uterine Nonactivated Estrogen Receptor to Nuclear Estrogen Receptor II
pp. 347-356(10)
Authors: Jaya P.; Varman Thampan R.

Type II Regulatory Subunit of cAMP-Dependent Protein Kinase: Purification Strategies to Optimize Crystallization
pp. 357-364(8)
Authors: Diller T.C.; Xuong N-H.; Taylor S.S.

Rational Design and Purification of Human Bruton's Tyrosine Kinase SH3–SH2 Protein for Structure–Function Studies
pp. 365-371(7)
Authors: Nera K-P.; Brockmann E.; Vihinen M.; Edvard Smith C.I.; Mattsson P.T.

A System for Dual Protein Expression in Pichia pastoris and Escherichia coli
pp. 372-378(7)
Authors: Lueking A.; Holz C.; Gotthold C.; Lehrach H.; Cahill D.

Production, Characterization, and Crystallization of Truncated Forms of Pneumococcal Surface Protein A from Escherichia coli
pp. 379-388(10)
Authors: Lamani E.; McPherson D.T.; Hollingshead S.K.; Jedrzejas M.J.

Cloning of Trypanosoma cruzi trans-Sialidase and Expression in Pichia pastoris
pp. 389-393(5)
Authors: Laroy W.; Contreras R.

Purification of GSK-3 by Affinity Chromatography on Immobilized Axin
pp. 394-404(11)
Authors: Primot A.; Baratte B.; Gompel M.; Borgne A.; Liabeuf S.; Romette J-L.; Jho E-h.; Costantini F.; Meijer L.

Expression, Purification, and Characterization of Deglycosylated Human Pro-Prostate-Specific Antigen
pp. 405-413(9)
Authors: Bowman K.K.; Clark J.; Yu L.; Mortara K.; Radika K.; Wang J.; Zhan H.

Prothymosin Is a Nonspecific Facilitator of Nuclear Processes: Studies of Run-on Transcription
pp. 414-420(7)
Authors: Trumbore M.W.; Berger S.L.

Synthesis of a Highly Substituted N⁶-Linked Immobilized NAD⁺ Derivative Using a Rapid Solid-Phase Modular Approach: Suitability for Use with the Kinetic Locking-on Tactic for Bioaffinity Purification of NAD⁺-Dependent Dehydrogenases
pp. 421-434(14)
Authors: Tynan J.; Forde J.; McMahon M.; Mulcahy P.

Coexpression of Proteins in Bacteria Using T7-Based Expression Plasmids: Expression of Heteromeric Cell-Cycle and Transcriptional Regulatory Complexes
pp. 435-443(9)
Authors: Johnston K.; Clements A.; Venkataramani R.N.; Trievel R.C.; Marmorstein R.

High Yield of Methylophilus methylotrophus Cytochrome c by Coexpression with Cytochrome c Maturation Gene Cluster from Escherichia coli
pp. 444-450(7)
Authors: Price N.J.; Brennan L.; Faria T.Q.; Vijgenboom E.; Canters G.W.; Turner D.L.; Santos H.

The Full-Length, Cytoplasmic C-Terminus of the 2-Adrenergic Receptor Expressed in E. coli Acts as a Substrate for Phosphorylation by Protein Kinase A, Insulin Receptor Tyrosine Kinase, GRK2, but Not Protein Kinase C and Suppresses Desensitization when Expressed in Vivo
pp. 451-461(11)
Authors: Doronin S.; Lin F.; Wang H-y.; Malbon C.C.

A Recombinant Group 1 House Dust Mite Allergen, rDer f 1, with Biological Activities Similar to Those of the Native Allergen
pp. 462-471(10)
Authors: Best E.A.; Stedman K.E.; Bozic C.M.; Hunter S.W.; Vailes L.; Chapman M.D.; McCall C.A.; McDermott M.J.

Expression and Characterization of Glycosylated and Catalytically Active Recombinant Human -Galactosidase A Produced in Pichia pastoris
pp. 472-484(13)
Authors: Chen Y.; Jin M.; Egborge T.; Coppola G.; Andre J.; Calhoun D.H.

Production of Full-Length Human Pre-elafin, an Elastase Specific Inhibitor, from Yeast Requires the Absence of a Functional Yapsin 1 (Yps1p) Endoprotease
pp. 485-491(7)
Authors: Bourbonnais Y.; Larouche C.; Tremblay G.M.

Expression, Refolding, and Isotopic Labeling of Human Serum Albumin Domains for NMR Spectroscopy
pp. 492-499(8)
Authors: Mao H.; Gunasekera A.H.; Fesik S.W.

A Mammalian Expression Vector for Expression and Purification of Secreted Proteins for Structural Studies
pp. 500-506(7)
Authors: Leahy D.J.; Dann C.E.; Longo P.; Perman B.; Ramyar K.X.

Recombinant Expression and Purification of Smad Proteins
pp. 507-513(7)
Authors: Funaba M.; Mathews L.S.

Reconstitution of the Vertebrate Visual Cascade Using Recombinant Heterotrimeric Transducin Purified from Sf9 Cells
pp. 514-526(13)
Authors: Min K.C.; Gravina S.A.; Sakmar T.P.

Author Index for Volume 20
pp. 527-528(2)

Cumulative Subject Index for Volumes 18–20
pp. 529-543(15)