Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. v-v(1)

Overexpression and Purification of Human XPA Using a Baculovirus Expression System
pp. 1-11(11)
Authors: Hermanson I.L.; Turchi J.J.

Generation of a Baculovirus Recombinant Prostate-Specific Membrane Antigen and Its Use in the Development of a Novel Protein Biochip Quantitative Immunoassay
pp. 12-21(10)
Authors: Xiao Z.; Jiang X.; Beckett M.L.; Wright G.L.

Expression and Purification of Recombinant Human Indoleamine 2,3-Dioxygenase
pp. 22-29(8)
Authors: Littlejohn T.K.; Takikawa O.; Skylas D.; Jamie J.F.; Walker M.J.; Truscott R.J.W.

Preparation of Recombinant Bovine, Porcine, and Porcine W4R/R5K Leptins and Comparison of Their Activity and Immunoreactivity with Ovine, Chicken, and Human Leptins
pp. 30-40(11)
Authors: Raver N.; Gussakovsky E.E.; Keisler D.H.; Krishna R.; Mistry J.; Gertler A.

Expression, Refolding, and Activity of a Recombinant Nonhemorrhagic Snake Venom Metalloprotease
pp. 41-47(7)
Authors: Selistre-de-Araujo H.S.; de Souza E.L.; Beltramini L.M.; Ownby C.L.; Souza D.H.F.

Overexpression and Purification of Fructose-1-Phosphate Kinase from Escherichia coli: Application to the Assay of Fructose 1-Phosphate
pp. 48-52(5)
Authors: Veiga-da-Cunha M.; Houyoux A.; Van Schaftingen E.

The Preparation of Apo-Cu,Zn Superoxide Dismutase by Ion-Exchange Chromatography on Iminodiacetic Acid–Sepharose
pp. 53-56(4)
Authors: Sutter B.; Bounds P.L.; Koppenol W.H.

Purification of Histidine-Tagged Mitochondrial ADP/ATP Carrier: Influence of the Conformational States of the C-Terminal Region
pp. 57-65(9)
Authors: Fiore C.; Trézéguet V.; Roux P.; Le Saux A.; Noël F.; Schwimmer C.; Arlot D.; Dianoux A-C.; Lauquin G.J.M.; Brandolin G.

Expression and Purification of Soluble and Inactive Mutant Forms of Membrane Type 1 Matrix Metalloproteinase
pp. 66-73(8)
Authors: Valtanen H.; Lehti K.; Lohi J.; Keski-Oja J.

Heterologous Expression in Pseudomonas aeruginosa and Purification of the 9.2-kDa c-Type Cytochrome Subunit of p-Cresol Methylhydroxylase
pp. 74-83(10)
Authors: Cronin C.N.; McIntire W.S.

Overexpression in Escherichia coli, Purification, and Characterization of Sphingomonas sp. A1 Alginate Lyases
pp. 84-90(7)
Authors: Yoon H-J.; Hashimoto W.; Miyake O.; Okamoto M.; Mikami B.; Murata K.

Functional Human Insulin-Degrading Enzyme Can Be Expressed in Bacteria
pp. 91-98(8)
Authors: Chesneau V.; Rosner M.R.

Active Recombinant Human Tyrosine Kinase c-Yes: Expression in Baculovirus System, Purification, Comparison to c-Src, and Inhibition by a c-Src Inhibitor
pp. 99-106(8)
Authors: ua M.; Luong-Nguyen N-H.; Crespo J.; Maier R.; Missbach M.; McMaster G.

Cloning, Expression, and Purification of the His₆-Tagged Thermostable -Galactosidase from Pyrococcus woesei in Escherichia coli and Some Properties of the Isolated Enzyme
pp. 107-112(6)
Authors: Dabrowski S.; Sobiewska G.; Maciuska J.; Synowiecki J.; Kur J.

Comparative Characterization of Two Forms of Recombinant Human SPC1 Secreted from Schneider 2 Cells
pp. 113-124(12)
Authors: Denault J-B.; Lazure C.; Day R.; Leduc R.

Cloning, Expression, and One-Step Purification of the Minimal Essential Domain of the Light Chain of Botulinum Neurotoxin Type A
pp. 125-130(6)
Authors: Kadkhodayan S.; Knapp M.S.; Schmidt J.J.; Fabes S.E.; Rupp B.; Balhorn R.

Production and Characterization of Biologically Active Human GM-CSF Secreted by Genetically Modified Plant Cells
pp. 131-138(8)
Authors: James E.A.; Wang C.; Wang Z.; Reeves R.; Shin J.H.; Magnuson N.S.; Lee J.M.

Yeast Cytochrome c Peroxidase Expression in Escherichia coli and Rapid Isolation of Various Highly Pure Holoenzymes
pp. 139-147(9)
Authors: Teske J.G.; Savenkova M.I.; Mauro J.M.; Erman J.E.; Satterlee J.D.

Folding and Structural Characterization of Highly Disulfide-Bonded Beetle Antifreeze Protein Produced in Bacteria
pp. 148-157(10)
Authors: Liou Y-C.; Daley M.E.; Graham L.A.; Kay C.M.; Walker V.K.; Sykes B.D.; Davies P.L.

Expression of Active Monomeric and Dimeric Nuclease A from the Gram-Positive Streptococcus gordonii Surface Protein Expression System
pp. 158-172(15)
Authors: Dutton E.K.; Ottum S.A.; Bolken T.C.; Franke C.A.; Hruby D.E.

High-Level Expression in Escherichia coli and Purification of the Membrane-Bound Form of Cytochrome b₅
pp. 173-178(6)
Authors: Mulrooney S.B.; Waskell L.

High-Level Production of Recombinant Fungal Endo--1,4-xylanase in the Methylotrophic Yeast Pichia pastoris
pp. 179-187(9)
Authors: Berrin J-G.; Williamson G.; Puigserver A.; Chaix J-C.; McLauchlan W.R.; Juge N.

Overexpression, Purification, and Crystallization of the Membrane-Bound Fumarate Reductase from Escherichia coli
pp. 188-196(9)
Authors: Luna-Chavez C.; Iverson T.M.; Rees D.C.; Cecchini G.

Endogenous Glutathione-Binding Proteins of Insect Cell Lines: Characterization and Removal from Glutathione S-Transferase (GST) Fusion Proteins
pp. 197-201(5)
Authors: Bichet P.; Mollat P.; Capdevila C.; Sarubbi E.

Purification and Characterization of Recombinant Human -N-Acetylglucosaminidase Secreted by Chinese Hamster Ovary Cells
pp. 202-211(10)
Authors: Zhao K-W.; Neufeld E.F.

Functional and Immunological Analysis of Recombinant Mouse H- and L-Ferritins from Escherichia coli
pp. 212-218(7)
Authors: Santambrogio P.; Cozzi A.; Levi S.; Rovida E.; Magni F.; Albertini A.; Arosio P.