Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. iv-iv(1)

Purification and Crystallization of Rhizopuspepsin: The Use of Nickel Chelation Chromatography to Select for Catalytically Active Species
pp. 213-220(8)
Authors: Flentke G.R.; Glinski J.; Satyshur K.; Rich D.H.

Purification of Human Chorionic Gonadotrophin from Urine by Membrane Filtration Affinity Chromatography with a Positively Charged Membrane
pp. 221-223(3)
Authors: Xu B.; Zhu Z.; Tang Y.; Huang Q.; Wang X.

Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
pp. 224-230(7)
Authors: Morrow J.A.; Arnold K.S.; Weisgraber K.H.

Purification of Xyloglucan Endotransglycosylase Based on Affinity Sorption of the Active Glycosyl–Enzyme Intermediate Complex to Cellulose
pp. 231-235(5)
Authors: Sulová Z.; Farka V.

Purification, Characterization, and Crystallization of the Distal BRCT Domain of the Human XRCC1 DNA Repair Protein
pp. 236-242(7)
Authors: Thornton K.; Forstner M.; Richard Shen M.; West M.G.; Rupp B.; Thelen M.P.

Expression and Characterization of Human Salivary Statherin from Escherichia coli Using Two Different Fusion Constructs
pp. 243-250(8)
Authors: Gilbert M.; Stayton P.S.

Heterologously Expressed Polypeptide from the Yeast Meiotic Gene HOP1 Binds Preferentially to Yeast DNA
pp. 251-260(10)
Authors: de Dios Alché J.; Paul E.; Dickinson H.

Effect of Accessible Immobilized NAD⁺ Concentration on the Bioaffinity Chromatographic Behavior of NAD⁺-Dependent Dehydrogenases Using the Kinetic Locking-on Strategy
pp. 261-275(15)
Authors: Mulcahy P.; O'Flaherty M.; McMahon M.; Oakey L.

The Kinetic Locking-on Strategy for Bioaffinity Purification: Further Studies with Bovine Liver Glutamate Dehydrogenase
pp. 276-297(22)
Authors: O'Flaherty M.; McMahon M.; Forde J.; Mulcahy P.

Expression and Purification of Recombinant Human Zona Pellucida Proteins
pp. 298-307(10)
Authors: Harris J.D.; Seid C.A.; Fontenot G.K.; Liu H.F.

Versatile Copurification Procedure for Rapid Isolation of Homogeneous RAR-RXR Heterodimers
pp. 308-314(7)
Authors: Iyer J.; Bonnier D.; Granger F.; Iltis C.; Andry V.; Schultz P.; Gronemeyer H.

Use of the Green Fluorescent Protein Variant (YFP) to Monitor MetArg Human Proinsulin Production in Escherichia coli
pp. 315-323(9)
Authors: Daabrowski S.; Brillowska A.; Kur J.

Expression of Human Gelatinase B in Pichia pastoris
pp. 324-330(7)
Authors: Roy N.; Padmanabhan S.; Smith M.; Shi L.; Navre M.; Das G.

Penicillin-Binding Protein 2a of Streptococcus pneumoniae: Expression in Escherichia coli and Purification and Refolding of Inclusion Bodies into a Soluble and Enzymatically Active Enzyme
pp. 331-339(9)
Authors: Zhao G.; Meier T.I.; Hoskins J.; Jaskunas S.R.

Purification of Granulin-like Polypeptide from the Blood-Sucking Leech, Hirudo nipponia
pp. 340-346(7)
Authors: Jin Hong S.; Won Kang K.

Production of Chemokines CTAPIII and NAP/2 by Digestion of Recombinant Ubiquitin–CTAPIII with Yeast Ubiquitin C-Terminal Hydrolase and Human Immunodeficiency Virus Protease
pp. 347-354(8)
Authors: Mildner A.M.; Paddock D.J.; LeCureux L.W.; Leone J.W.; Anderson D.C.; Tomasselli A.G.; Heinrikson R.L.

A Modified Procedure for Fast Purification of T7 RNA Polymerase
pp. 355-358(4)
Authors: Li Y.; Wang E.; Wang Y.

High-Level Expression and Purification of Biologically Active Recombinant Pokeweed Antiviral Protein
pp. 359-368(10)
Authors: Rajamohan F.; Engstrom C.R.; Denton T.J.; Engen L.A.; Kourinov I.; Uckun F.M.