Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. v-v(1)

AnEscherichia coliExpression Vector That Allows Recovery of Proteins with Native N-Termini from Purified Calmodulin-Binding Peptide Fusions
pp. 1-10(10)
Authors: Wyborski D.L.; Bauer J.C.; Zheng C-F.; Felts K.; Vaillancourt P.

Kinetic Properties of Human Dopamine Sulfotransferase (SULT1A3) Expressed in Prokaryotic and Eukaryotic Systems: Comparison with the Recombinant Enzyme Purified fromEscherichia coli
pp. 11-18(8)
Authors: Dajani R.; Sharp S.; Graham S.; Bethell S.S.; Cooke R.M.; Jamieson D.J.; Coughtrie M.W.H.

Soluble Expression inEscherichia coliof Murine Endogenous Retroviral Transmembrane Envelope Protein Having Immunosuppressive Activity
pp. 19-26(8)
Authors: Kim K.S.; Kim K.H.; Choi S.E.; Yoon J-W.; Kang Y.

Site-Directed Mutagenesis of Human Dihydrolipoamide Dehydrogenase: Role of Lysine-54 and Glutamate-192 in Stabilizing the Thiolate–FAD Intermediate
pp. 27-39(13)
Authors: Liu T-C.; Soo Hong Y.; Korotchkina L.G.; Vettakkorumakankav N.N.; Patel M.S.

Circular Dichroism Analysis of Insect Cell Expressed Herpes Simplex Virus Type I Single-Stranded DNA-Binding Protein ICP8
pp. 40-46(7)
Authors: Spatz M.; Ali S.A.; Auer M.; Graf C.; Eibl M.M.; Steinkasserer A.

Purification of Bovine S100A12 from RecombinantEscherichia coli
pp. 47-52(6)
Authors: Yamashita K.; Oyama Y.; Shishibori T.; Matsushita O.; Okabe A.; Kobayashi R.

Stable, High-Level Expression of a Type I Antifreeze Protein inEscherichia coli
pp. 53-62(10)
Authors: Solomon R.G.; Appels R.

pSKAP/S: An Expression Vector for the Production of Single-Chain Fv Alkaline Phosphatase Fusion Proteins
pp. 63-69(7)
Authors: Griep R.A.; van Twisk C.; Kerschbaumer R.J.; Harper K.; Torrance L.; Himmler G.; van der Wolf J.M.; Schots A.

Expression of a Lipocalin in Prokaryote and Eukaryote Cells: Quantification and Structural Characterization of Recombinant Bovine -Lactoglobulin
pp. 70-75(6)
Authors: Chatel J-M.; Adel-Patient K.; Créminon C.; Wal J-M.

Purification of Active Matrix Metalloproteinase Catalytic Domains and Its Use for Screening of Specific Stromelysin-3 Inhibitors
pp. 76-83(8)
Authors: Kannan R.; Ruff M.; Kochins J.G.; Manly S.P.; Stoll I.; El Fahime M.; Noël A.; Foidart J-M.; Rio M-C.; Dive V.; Basset P.

Bacterial Expression and Purification of the Fab Fragment of a Monoclonal Antibody Specific for the Low-Density Lipoprotein Receptor-Binding Site of Human Apolipoprotein E
pp. 84-90(7)
Authors: Raffaï R.; Vukmirica J.; Weisgraber K.H.; Rassart E.; Innerarity T.L.; Milne R.

Thein SituRegeneration and Extraction of Recombinant Aequorin fromEscherichia coliCells and the Purification of Extracted Aequorin
pp. 91-95(5)
Authors: Shimomura O.; Inouye S.

Cloning, Overexpression, and Purification of the Recombinant His-Tagged SSB Protein ofEscherichia coliand Use in Polymerase Chain Reaction Amplification
pp. 96-102(7)
Authors: Dbrowski S.; Kur J.

Soluble Expression of Cloned Phage K11 RNA Polymerase Gene inEscherichia coliat a Low Temperature
pp. 103-108(6)
Authors: Han K.G.; Lee S.S.; Kang C.

Application of Fed-Batch Fermentation to the Preparation of Isotopically Labeled or Selenomethionyl-Labeled Proteins
pp. 109-119(11)
Authors: Studts J.M.; Fox B.G.

Saccharomyces cerevisiaeCells Harboring the Gene Encoding Sarcotoxin IA Secrete a Peptide That Is Toxic to Plant Pathogenic Bacteria
pp. 120-124(5)
Authors: Aly R.; Granot D.; Mahler-Slasky Y.; Halpern N.; Nir D.; Galun E.

Heterologous Expression of 5-Methylthioadenosine Phosphorylase from the ArchaeonSulfolobus solfataricus:Characterization of the Recombinant Protein and Involvement of Disulfide Bonds in Thermophilicity and Thermostability
pp. 125-135(11)
Authors: Cacciapuoti G.; Fusco S.; Caiazzo N.; Zappia V.; Porcelli M.

Expression of Human Plasminogen inDrosophilaSchneider S2 Cells
pp. 136-143(8)
Authors: Nilsen S.L.; Castellino F.J.

Recombinant Production of the p10^CKS1AtProtein fromArabidopsis thalianaand¹³C and¹⁵N Double-Isotopic Enrichment for NMR Studies
pp. 144-151(8)
Authors: Landrieu I.; Casteels P.; Odaert B.; De Veylder L.; Portetelle D.; Lippens G.; Van Montagu M.; Inzé D.

Recombinant Expression of a Galectin From the Sheep Gastrointestinal ParasiteTeladorsagia circumcincta:Its Use in Isolating Galectin-Glycoconjugates
pp. 152-159(8)
Authors: Greenhalgh C.J.; Newton S.E.

Folding and Purification of a Recombinantly Expressed Interferon Regulatory Factor, IRF-4
pp. 160-170(11)
Authors: Moellering B.J.; Yoshinaga S.K.; Hui A.; Delaney J.M.; Hara S.; Narhi L.O.; Westcott K.R.

Expression, Purification, and Characterization of the Recombinant Calcium-Binding Equine Lysozyme Secreted by the Filamentous FungusAspergillus niger:Comparisons with the Production of Hen and Human Lysozymes
pp. 171-180(10)
Authors: Spencer A.; Morozov-Roche L.A.; Noppe W.; MacKenzie D.A.; Jeenes D.J.; Joniau M.; Dobson C.M.; Archer D.B.

Renaturation ofEscherichia coli-Derived Recombinant Human Macrophage Colony-Stimulating Factor
pp. 181-189(9)
Authors: Tran-Moseman A.; Schauer N.; De Bernardez Clark E.

High-Level Expression and Purification of the Recombinant Diphtheria Fusion Toxin DTGM for PHASE I Clinical Trials
pp. 190-201(12)
Authors: Frankel A.E.; Ramage J.; Latimer A.; Feely T.; Delatte S.; Hall P.; Tagge E.; Kreitman R.; Willingham M.

Adenoviral-Mediated Expression of Human Insulin-like Growth Factor-Binding Protein-3
pp. 202-211(10)
Authors: Firth S.M.; Ganeshprasad U.; Poronnik P.; Cook D.I.; Baxter R.C.