Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. iv-iv(0)

Purification of Active Cysteine Proteases by Affinity Chromatography with Attached E-64 Inhibitor
pp. 247-250(4)
Authors: Govrin E.; Levine A.

Expression and Characterization of Recombinant Mast Cell Tryptase
pp. 251-257(7)
Authors: Chan H.; Elrod K.C.; Numerof R.P.; Sideris S.; Clark J.M.

Overexpression and Purification of an Immunologically Reactive His–BIV Capsid Fusion Protein
pp. 258-264(7)
Authors: Betemps D.; Mallet F.; Cheynet V.; Baron T.

Expression and Purification of Human Calbindin D_28k
pp. 265-270(6)
Authors: Thulin E.; Linse S.

Expression and Characterization of the Extracellular Domain of Guanylyl Cyclase C from a Baculovirus and Sf21 Insect Cells
pp. 271-281(11)
Authors: Hasegawa M.; Kawano Y.; Matsumoto Y.; Hidaka Y.; Fujii J-I.; Taniguchi N.; Wada A.; Hirayama T.; Shimonishi Y.

Purification and Characterization of a Metalloprotease from Chryseobacterium indologenes Ix9a and Determination of the Amino Acid Specificity with Electrospray Mass Spectrometry
pp. 282-295(14)
Authors: Venter H.; Osthoff G.; Litthauer D.

Differences in the Expression and Localization of Human Melanotransferrin in Lepidopteran and Dipteran Insect Cell Lines
pp. 296-307(12)
Authors: Hegedus D.D.; Pfeifer T.A.; Theilmann D.A.; Kennard M.L.; Gabathuler R.; Jefferies W.A.; Grigliatti T.A.

Streamlined Procedure for the Production of Normal and Altered Versions of Recombinant Human Proinsulin
pp. 308-313(6)
Author: Mackin R.B.

Spinach Holo-Acyl Carrier Protein: Overproduction and Phosphopantetheinylation in Escherichia coli BL21(DE3), in Vitro Acylation, and Enzymatic Desaturation of Histidine-Tagged Isoform I
pp. 314-326(13)
Authors: Broadwater J.A.; Fox B.G.

High-Level Expression of Rhizopus niveus Lipase in the Yeast Saccharomyces cerevisiae and Structural Properties of the Expressed Enzyme
pp. 327-335(9)
Authors: Kohno M.; Enatsu M.; Yoshiizumi M.; Kugimiya W.

Escherichia coli Skp Chaperone Coexpression Improves Solubility and Phage Display of Single-Chain Antibody Fragments
pp. 336-343(8)
Authors: Hayhurst A.; Harris W.J.

Purification and Stabilization of a Monomeric Isocitrate Dehydrogenase from Corynebacterium glutamicum
pp. 344-348(5)
Authors: Bai C.; Fernandez E.; Yang H.; Chen R.

The Expression of Recombinant Large Myelin-Associated Glycoprotein Cytoplasmic Domain and the Purification of Native Myelin-Associated Glycoprotein from Rat Brain and Peripheral Nerve
pp. 349-361(13)
Authors: Heape A.M.; Lehto V-P.; Kursula P.

Optimization of the Production of a Honeybee Odorant-Binding Protein by Pichia pastoris
pp. 362-369(8)
Authors: Briand L.; Perez V.; Huet J-C.; Danty E.; Masson C.; Pernollet J-C.

Expression and One-Step Purification of a Fully Active Polyhistidine-Tagged Cytochrome bc₁ Complex from Rhodobacter sphaeroides
pp. 370-380(11)
Authors: Guergova-Kuras M.; Salcedo-Hernandez R.; Bechmann G.; Kuras R.; Gennis R.B.; Crofts A.R.

Production, Purification, and Luminometric Analysis of Recombinant Saccharomyces cerevisiae MET3 Adenosine Triphosphate Sulfurylase Expressed in Escherichia coli
pp. 381-388(8)
Authors: Karamohamed S.; Nilsson J.; Nourizad K.; Ronaghi M.; Pettersson B.; Nyrén P.

Removal of N-Terminal Polyhistidine Tags from Recombinant Proteins Using Engineered Aminopeptidases
pp. 389-400(12)
Authors: Pedersen J.; Lauritzen C.; Madsen M.T.; Weis Dahl S.

Author Index for Volume 15
pp. 401-402(2)