Protein Expression and Purification

Papers to Appear in Forthcoming Issues
pp. iv-iv(1)

A Method for the Purification and Refolding of a Recombinant Form of the Nontypeable Haemophilus influenzae P5 Outer Membrane Protein Fused to Polyhistidine
pp. 1-7(7)
Authors: Webb D.C.; Cripps A.W.

Cloning, Expression, and Purification of Cytidine Deaminase from Arabidopsis thaliana
pp. 8-15(8)
Authors: Vincenzetti S.; Cambi A.; Neuhard J.; Schnorr K.; Grelloni M.; Vita A.

Expression, Folding, and Characterization of Small Proteins with Increasing Disulfide Complexity by a pT7-7-Derived Phagemid
pp. 16-23(8)
Authors: Peterson F.C.; Anderson P.J.; Berliner L.J.; Brooks C.L.

Production of Reagents and Optimization of Methods for Studying Calmodulin-Binding Proteins
pp. 24-33(10)
Authors: Ulbricht B.; Soldati T.

Overcoming Expression and Purification Problems of RhoGDI Using a Family of “Parallel” Expression Vectors
pp. 34-39(6)
Authors: Sheffield P.; Garrard S.; Derewenda Z.

Overexpression in Escherichia coli, Purification, and Characterization of Recombinant 60S Ribosomal Acidic Proteins from Saccharomyces cerevisiae
pp. 40-47(8)
Authors: Tchórzewski M.; Boguszewska A.; Abramczyk D.; Grankowski N.

Purification of a Multicatalytic Protease Complex from Developing Winged Bean Seeds by Indirect Immunoaffinity Chromatography
pp. 48-56(9)
Authors: Usha R.; Singh M.

Purified Recombinant Escherichia coli Ketol-Acid Reductoisomerase Is Unsuitable for Use in a Coupled Assay of Acetohydroxyacid Synthase Activity due to an Unexpected Side Reaction
pp. 57-61(5)
Authors: Hill C.M.; Duggleby R.G.

Large-Scale Propagation of Recombinant Adherent Cells That Secrete a Stable Form of Human Glandular Kallikrein, hK2
pp. 62-68(7)
Authors: Kumar A.; Goel A.S.; Payne J.K.; Evans C.; Mikolajczyk S.D.; Kuus-Reichel K.; Saedi M.S.

Expression, Purification, and Characterization of Recombinant Forms of Membrane-Bound Cytochrome c-550nm from Bacillus subtilis
pp. 69-76(8)
Authors: David P.S.; Morrison M.R.; Wong S-L.; Hill B.C.

Disulfide Bond Formation and Folding of Plant Peroxidases Expressed as Inclusion Body Protein in Escherichia coli Thioredoxin Reductase Negative Strains
pp. 77-82(6)
Authors: Teilum K.; Østergaard L.; Welinder K.G.

Construction of Phosphorylatable Chimeric Monoclonal Antibody CC49 with a Casein Kinase I Recognition Site
pp. 83-91(9)
Authors: Lin L.; Gillies S.D.; Schlom J.; Pestka S.

Optimized Heterologous Expression of Glutathione Reductase from Cyanobacterium Anabaena PCC 7120 and Characterization of the Recombinant Protein
pp. 92-98(7)
Authors: Jiang F.; Mannervik B.

The Refolding, Purification, and Activity Analysis of a Rice Bowman–Birk Inhibitor Expressed in Escherichia coli
pp. 99-104(6)
Authors: Li N.; Qu L-J.; Liu Y.; Li Q.; Gu H.; Chen Z.

Empty and Peptide-Loaded Class II Major Histocompatibility Complex Proteins Produced by Expression in Escherichia coli and Folding in Vitro
pp. 105-114(10)
Authors: Frayser M.; Sato A.K.; Xu L.; Stern L.J.

High-Level Expression and Single-Step Purification of Leucyl-tRNA Synthetase from Escherichia coli
pp. 115-120(6)
Authors: Chen J.; Li Y.; Wang E.; Wang Y.

Large-Scale Expression of Recombinant Cardiac Sodium–Calcium Exchange in Insect Larvae
pp. 121-126(6)
Authors: Hale C.C.; Zimmerschied J.A.; Bliler S.; Price E.M.

A Kinetic Locking-On Strategy for Bioaffinity Purification: Further Studies with Alcohol Dehydrogenase
pp. 127-145(19)
Authors: O'Flaherty M.; McMahon M.; Mulcahy P.

Design and Expression of a Synthetic Mucin Gene Fragment in Escherichia Coli
pp. 146-154(9)
Authors: Dolby N.; Dombrowski K.E.; Wright S.E.