IngentaConnect Expression of pepper cyclophilin gene is differentially regulated...

Authors: Kong H.Y.; Lee S.C.; Hwang B.K.

Source: Physiological and Molecular Plant Pathology, Volume 59, Number 4, October 2001 , pp. 189-199(11)

Abstract:

A pathogen induced pepper cyclophilin (CACYP1) cDNA clone was isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with Xanthomonas campestris pv.vesicatoria using a differential hybridization technique. The deduced amino acid sequences of thisCACYP1gene have high levels of identity with those of cyclophilin genes from tomatoes, potatoes, beans andArabidopsis. The CACYP1 mRNA was constitutively expressed in the leaf, stem, root and flower, but not in the green (unripe) and the red (ripe) fruits of pepper plants. The accumulation of CACYP1 mRNA was strongly induced in pepper plants by X. campestris pv. vesicatoria and Colletotrichum gloeosporioidesinfections. In the incompatible interaction, a drastic induction of the CACYP1 mRNA occurred in the bacteria inoculated leaves compared to that of the compatible interaction. Prior inoculation with an avirulent strain of X. campestris pv.vesicatoria in lower leaves resulted in a strong, systemic CACYP1 mRNA accumulation in the uninoculated upper leaves. In situ hybridization results revealed that CACYP1 mRNA was localized in the phloem cells of vascular tissues in pepper fruits infected by C. gloeosporioides. Treatment with salicyclic acid and methyl jasmonate strongly induced the CACYP1 transcripts in pepper leaves. Copyright 2001 Academic Press

Keywords: cyclophilin; pathogen; abiotic stress; Capsicum annuum

Language: English

Document Type: Research article

Affiliations: Laboratory of Molecular Plant Pathology, College of Life and Environmental Sciences, Korea University, Seoul, 136-701, Korea:

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Expression of pepper cyclophilin gene is differentially regulated during the pathogen infection and abiotic stress conditions