Nucleotide Sequence and Analysis of pBL1, a Bacteriocin-Producing Plasmid from Lactococcus lactis IPLA 972
Authors: Sánchez C.1; Hernández de Rojas A.1; Martínez B.1; Argüelles M.E.1; Suárez J.E.1, 2; Rodríguez A.1; Mayo B.1
Source: Plasmid, Volume 44, Number 3, November 2000 , pp. 239-249(11)
Publisher: Academic Press
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Abstract:
The complete sequence of the 10.9-kbp bacteriocinogenic plasmid pBL1 from Lactococcus lactis subsp. lactis IPLA 972 has been determined. Thirteen ORFs were encountered, of which 5 were incomplete. pBL1 proved to be a narrow-host-range plasmid which replicates neither in Bacilus subtilis nor in Lactobacillus spp. The structural organization of the pBL1 replication region was highly similar to other well-known theta-replicating plasmids of lactococci, at both the untranslated (the replication origin) and the translated (repB and orfX) sequences. As in other plasmids, the product of orfX was not necessary for plasmid replication. However, it was shown to be involved in plasmid stability. Three genes organized in an operon-like structure encompassed, most likely, the bacteriocin-encoding region. Upstream of the origin of replication a nicking site (oriT) was found. This oriT sequence proved to be functional by mobilization of plasmids wearing it. One complete and several partial IS elements were identified on pBL1. Copyright 2000 Academic Press.
Keywords: Lactococcus lactis; bacteriocin; plasmid; DNA sequence; origin of replication; repB; oriT.
Language: English
Document Type: Research article
Affiliations: 1: Instituto de Productos Lácteos de Asturias (CSIC), Carretera de Infiesto s/n, Villaviciosa, Asturias, 33300-, Spain 2: Departamento de Biología Funcional, Universidad de Oviedo, Julián Clavería s/n, Oviedo, Asturias, 33006-, Spain
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