Functional Comparison of Conjugative Transposons Tn 916 and Tn 925

Authors: Showsh S.A.; Andrews Jr R.E.

Source: Plasmid, Volume 35, Number 3, May 1996 , pp. 164-173(10)

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Abstract:

During interspecies matings between Bacillus subtilis and Bacillus thuringiensis subsp. israelensis, transfer of conjugative transposon Tn 916 was detected at a frequency of 1.1 x 10 -4 transconjugants per donor. Tn 916 -dependent transfer of plasmids pC194 and pE194 was detected at frequencies of 1.4 x 10 -5 and 3.2 x 10 -7 transconjugants per donor, respectively. Similar frequencies were obtained during parallel matings with otherwise isogenic strains that contain Tn 925 instead of Tn 916 . Tn 916 - or Tn 925 -dependent transfer of plasmids pC194 or pUB110 from the recipient to the donor (retrotransfer) was not observed during inter- or intraspecies matings. Transposon-mediated plasmid transfer by Tn 916 and Tn 925 is a Rec independent event. Thus, the data from studies in which otherwise isogenic donor and recipient strains were used indicated that Tn 916 and Tn 925 are, from a functional point of view, much more similar than previously suggested.

Language: English

Document Type: Research article

Affiliations: Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University, Ames, Iowa, 50011

Publication date: 1996-05-01