Altered Hippocampal Kainate-Receptor mRNA Levels in Temporal Lobe Epilepsy Patients

Authors: Mathern G.W.^{1, 4, 5}; Pretorius J.K.⁵; Kornblum H.I.^{5, 2, 3}; Mendoza D.⁵; Lozada A.⁵; Leite J.P.⁷; Chimelli L.⁶; Born D.E.¹⁰; Fried I.^{1, 5}; Sakamoto A.C.⁷; Assirati J.A.⁸; Peacock W.J.¹; Ojemann G.A.¹¹; Adelson P.D.⁹

Source: Neurobiology of Disease, Volume 5, Number 3, September 1998 , pp. 151-176(26)

Publisher: Academic Press

Abstract:

This study determined whether hippocampal kainate (KA) receptor mRNA levels were increased or decreased in temporal lobe epilepsy patients compared with nonseizure autopsies. Hippocampal sclerosis (HS; n = 17), nonsclerosis (non-HS; n = 11), and autopsy hippocampi (n = 9) were studied for KA1–2 and GluR5–7 mRNA levels using semiquantitative in situ hybridization techniques, along with neuron densities. Compared with autopsy hippocampi, HS and non-HS cases showed decreased GluR5 and GluR6 hybridization densities per CA2 and/or CA3 pyramid. Furthermore, HS patients demonstrated increased KA2 and GluR5 hybridization densities per granule cell compared with autopsy hippocampi. These findings indicate that chronic temporal lobe seizures were associated with differential changes in hippocampal KA1–2 and GluR5–7 hybridization densities that vary by subfield and pathology group. In temporal lobe epilepsy patients, these results support the hypothesis that pyramidal cell GluR5 and GluR6 mRNA levels are decreased as a consequence of seizures, and in HS patients granule cell KA2 and GluR5 mRNA levels are increased in association with aberrant fascia dentata mossy fiber sprouting and/or hippocampal neuronal loss. Copyright 1998 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: Division of Neurosurgery 10: Department of Pathology–Neuropathology and 11: Department of Pathology–Neuropathology and, Department of Neurosurgery, University of Washington School of Medicine, Seattle, Washington, 98195 2: Division of Neurosurgery, Department of Pediatrics 3: Division of Neurosurgery, Department of Pediatrics, Department of Molecular and Medical Pharmacology 4: Division of Neurosurgery, Department of Pediatrics, Department of Molecular and Medical Pharmacology, Mental Retardation Research Center 5: Division of Neurosurgery, Department of Pediatrics, Department of Molecular and Medical Pharmacology, Mental Retardation Research Center, The Brain Research Institute, University of California, Los Angeles, Los Angeles, California, 90095 6: Department of Pathology 7: Department of Pathology, Department of Neurology 8: Department of Pathology, Department of Neurology, Department of Neurosurgery, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, 15213, Brazil (SP) 9: Department of Neurosurgery, University of Pittsburgh, Pittsburgh, Pennsylvania

Publication date: 1998-09-01

• In this: publication
• By this: publisher
• In this Subject: Neurology & Psychiatry
• By this author: Mathern G.W. ;  Pretorius J.K. ;  Kornblum H.I. ;  Mendoza D. ;  Lozada A. ;  Leite J.P. ;  Chimelli L. ;  Born D.E. ;  Fried I. ;  Sakamoto A.C. ;  Assirati J.A. ;  Peacock W.J. ;  Ojemann G.A. ;  Adelson P.D.

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