13C Selective Polarization and Spin Diffusion in a Lipid Bilayer-Bound Polypeptide by Solid-State NMR
Source: Journal of Magnetic Resonance, Volume 139, Number 2, August 1999 , pp. 377-381(5)
Publisher: Academic Press
While 15N solid-state NMR has proven to be very advantageous for the development of structural biological methods, 13C spectroscopy has increased sensitivity and spectral dispersion. However, large natural abundance signals and homonuclear dipolar interactions pose significant problems. Here we have used a pair of 13C-labeled sites in a lipid-solubilized polypeptide to show the selective polarization can be used in combination with spin diffusion to achieve simplified spectra. Both unoriented and oriented samples have been used, with the latter providing a well-resolved homonuclear dipolar splitting. Copyright 1999 Academic Press.
Document Type: Research article
Affiliations: 1: Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory 2: Center for Interdisciplinary Magnetic Resonance, National High Magnetic Field Laboratory, Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida, 32310
Publication date: 1999-08-01