Anti-Glomerular Basement Membrane Disease: Role of Enzyme-Linked Immunosorbent Assays in Diagnosis
Authors: Litwin C.M.1, 3; Mouritsen C.L.3; Wilfahrt P.A.4; Schroder M.C.3; Hill H.R.1, 3, 2
Source: Biochemical and Molecular Medicine, Volume 59, Number 1, October 1996 , pp. 52-56(5)
Publisher: Academic Press
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Abstract:
We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivity, 97.0% specificity). The second ELISA kit was much less sensitive (87.8% agreement, 63.3% sensitivity, 92.2% specificity). ELISA tests for the measurement of anti-GBM antibodies allow for quantitation and may have greater sensitivity than IFA. Sensitivity, however, may depend on the commercial test used.
Language: English
Document Type: Research article
Affiliations: 1: Section of Clinical Immunology, Microbiology and Virology, Division of Clinical Pathology, Department of Pathology, University of Utah, Salt Lake City, Utah, 84132 2: Department of Pediatrics, University of Utah, Salt Lake City, Utah, 84132 3: Laboratory of Immunology and Infectious Diseases, Associated Regional and University Pathologists, Salt Lake City, Utah 4: Department of Pathology, University of Utah, Salt Lake City, Utah, 84132
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