Bacteriophage T4 Development in Escherichia coli is Growth Rate Dependent

Authors: RABINOVITCH A.¹; FISHOV I.²; HADAS H.²; EINAV M.²; ZARITSKY A.²

Source: Journal of Theoretical Biology, Volume 216, Number 1, May 2002 , pp. 1-4(4)

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Abstract:

Three independent parameters (eclipse and latent periods, and rate of ripening during the rise period) are essential and sufficient to describe bacteriophage development in its bacterial host. A general model to describe the classical “one-step growth” experiment [Rabinovitch et al. (1999a) J. Bacteriol.181,1687–1683] allowed their calculations from experimental results obtained with T4 in Escherichia coli B/r under different growth conditions [Hadas et al. (1997) Microbiology143, 179–185]. It is found that all three parameters could be described by their dependence solely on the culture doubling time tau before infection. Their functional dependence on tau , derived by a best-fit analysis, was used to calculate burst size values. The latter agree well with the experimental results. The dependence of the derived parameters on growth conditions can be used to predict phage development under other experimental manipulations. Copyright 2002 Elsevier Science Ltd. All rights reserved.

Language: English

Document Type: Research article

Affiliations: 1: Departments of Physics, Ben-Gurion University of the Negev, Be'er-Sheva, 84105, Israel 2: Ben-Gurion University of the Negev, Be'er-Sheva, 84105, Israel

Publication date: 2002-05-01