IngentaConnect Structure and Expression of the Gene Encoding Murine M-Protein, a...

Authors: Steiner F.^{1, 2}; Weber K.¹; Furst D.O.²

Source: Genomics, Volume 49, Number 1, April 1998 , pp. 83-95(13)

Abstract:

The complete exon-intron organization of the murine gene encoding M-protein, a structural protein of sarcomeric myofibrils, was determined. The gene is composed of 37 exons and 36 introns, spanning =~75 kb of DNA. Intron positions are related to the modular structure of M-protein, which is composed essentially of immunoglobulin and fibronectin type III domains. Almost all repeats follow a two exon-one domain structure. The beginning and end of each domain are defined by introns in phase I; internal introns are more divergent in position and very rarely use phase I. A single transcriptional start point was detected in both skeletal and cardiac muscle. Analysis of the prospective promoter region revealed several potential regulatory elements. CAT expression assays using promoter deletion constructs identified three regions that seem to be most important for the muscle-specific transcription activation of the M-protein gene. These results provide the first complete characterization of a gene for a member of the intracellular branch of the immunoglobulin superfamily. Copyright 1998 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: Department of Biochemistry, Max-Planck-Institute for Biophysical Chemistry, Gottingen, D-37077, Germany 2: Department of Cell Biology, University of Potsdam, Lennestrasse 7a, Potsdam, D-14471, Germany

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Structure and Expression of the Gene Encoding Murine M-Protein, a Sarcomere-Specific Member of the Immunoglobulin Superfamily