A Methylation Imprint Mark in the Mouse Imprinted Gene Grf1/Cdc25Mm Locus Shares a Common Feature with the U2afbp-rs Gene: An Association with a Short Tandem Repeat and a Hypermethylated Region

Authors: Shibata H.¹; Yoda Y.²; Kato R.³; Ueda T.⁴; Kamiya M.¹; Hiraiwa N.²; Yoshiki A.²; Plass C.⁵; Pearsall R.S.⁵; Held W.A.⁵; Muramatsu M.¹; Sasaki H.³; Kusakabe M.²; Hayashizaki Y.¹

Source: Genomics, Volume 49, Number 1, April 1998 , pp. 30-37(8)

Publisher: Academic Press

Abstract:

We identified a sperm-specific methylation imprint mark (Site II) associated with a short tandem repeat sequence and a site/region methylated in both gametes (Site I) in the Grf1 locus on mouse chromosome 9, which shared a common feature with the U2afbp-rs gene. Sites or regions of gamete-specific methylation in imprinted genes are strong candidates for carrying information regarding the parental origin of alleles. The gamete-specific methylation pattern of Sites I and II was conserved after fertilization, but attained the somatic cell pattern by the blastocyst stage. In primordial germ cells, Site I was methylated, but Site II was unmethylated in both male and female embryos, suggesting that the sperm-specific methylation imprint mark in Site II was established during spermatogenesis. These common features in methylation imprint regions may be a clue to identifying regions carrying primary information for the imprinting regulation. Copyright 1998 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: Genome Science Laboratory 2: Genome Science Laboratory, Division of Experimental Animal Research, RIKEN Tsukuba Life Science Center, 3-1-1 Koyadai, Ibaraki, 305, Japan 3: Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Fukuoka, 812-82, Japan 4: Research Laboratory for Molecular Genetics, Niigata University, 757 Ichiban-chou, Niigata, 951, Japan 5: Molecular and Cellular Biology Department, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, New York, 14263

Publication date: 1998-04-01

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• In this Subject: Biology ,  Biotechnology ,  Genetics
• By this author: Shibata H. ;  Yoda Y. ;  Kato R. ;  Ueda T. ;  Kamiya M. ;  Hiraiwa N. ;  Yoshiki A. ;  Plass C. ;  Pearsall R.S. ;  Held W.A. ;  Muramatsu M. ;  Sasaki H. ;  Kusakabe M. ;  Hayashizaki Y.

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