IngentaConnect Characterization of Human and Mouse Rod cGMP Phosphodiesterase de...

Authors: Li N.^{1, 2}; Florio S.K.³; Pettenati M.J.⁴; Rao P.N.⁴; Beavo J.A.³; Baehr W.¹

Source: Genomics, Volume 49, Number 1, April 1998 , pp. 76-82(7)

Abstract:

The mammalian multisubunit photoreceptor cGMP phosphodiesterase PDEalphabetagamma 2 (PDE6 family) is a peripherally membrane-associated enzyme. A novel subunit, termed PDEdelta (HGMW-approved symbol, PDE6D; MW 17 kDa), is able to detach PDE partially from bovine rod outer segment membranes under physiological conditions. Cloning of human and mouse PDEdelta cDNAs revealed that PDEdelta is a nearly perfectly conserved polypeptide of 150 amino acids that shows partial sequence homology to photoreceptor RG4 of unknown function. Multiple-species Southern blot analysis demonstrates that the PDEdelta gene has been well conserved during evolution and is detectable at high stringency in invertebrates. The human and mouse genes are contained in less than 8 kb of genomic DNA and consist of four exons and three introns (0.7-4 kb in human, 0.7-2.2 kb in mouse). The PDEdelta gene structure is identical to that of the C27H5.1 gene identified in the eyeless nematode Caenorhabditis elegans. The human PDEdelta gene (locus designation PDE6D) was localized to the long arm of chromosome 2 (2q35-q36) by fluorescence in situ hybridization. By synteny, the mouse PDEdelta gene is predicted to reside on chromosome 1. Copyright 1998 Academic Press.

Language: English

Document Type: Research article

Affiliations: 1: Moran Eye Center, University of Utah Health Center, Salt Lake City, Utah, 84132 2: Department of Biochemistry, Baylor College of Medicine, Houston, Texas, 77030 3: Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington, 98195 4: Section on Medical Genetics, Bowman Gray School of Medicine, Winston-Salem, North Carolina, 27157

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Characterization of Human and Mouse Rod cGMP Phosphodiesterase delta Subunit (PDE6D) and Chromosomal Localization of the Human Gene