IngentaConnect Extraction and Identification of Antioxidative Components of Hsia...

Authors: Hung C-Y.; Yen G-C.

Source: Lebensmittel-Wissenschaft und-Technologie, Volume 34, Number 5, August 2001 , pp. 306-311(6)

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Abstract:

The antioxidative activities of various solvent extracts of Hsian-tsao (Mesona procumbensHemsl.) were investigated. The results showed that for the same concentration of 0.2 g/kg, the antioxidative activities of various Hsian-tsao extracts, especially the ethyl acetate extract, which exhibited 93% inhibition of peroxidation of linoleic acid, were greater than those of BHA and -tocopherol. When the ethyl acetate extract of Hsian-tsao (EEHT) was separated into nine fractions by silica gel chromatography, fraction II showed high yield and high antioxidative activity. Fraction II was further separated into five subfractions using silica gel chromatography. Subfraction IIC was then isolated by preparative HPLC equipped with a silica column and four components were identified as stigmasterol, -sitosterol, oleanolic acid, and ursolic acid by UV, EI-MS,¹H-, and¹³SC-NMR. The descending order of antioxidative activity for those compounds was: EEHT>BHA> -tocopherol>ursolic acid>oleanolic acid>-sitosterol>stigmasterol. Copyright 2001 Academic Press.

Keywords: Mesona procumbens Hemsl; antioxidant activity; stigmasterol; -sitosterol; oleanolic acid; ursolic acid

Language: English

Document Type: Research article

Affiliations: Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung, Taiwan, 402, Republic of China:

Extraction and Identification of Antioxidative Components of Hsian-tsao (Mesona procumbens Hemsl.)