EXPERIMENTAL APPROACHES FOR OBSERVING HOMOLOGOUS DESENSITISATION AND THEIR PITFALLS

Authors: MODEL M.A.¹; OMANN G.M.²

Source: Pharmacological Research, Volume 38, Number 1, July 1998 , pp. 41-44(4)

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Abstract:

Whereas molecular mechanisms of cell desensitisation have been discussed at length in the literature, little organised information on the methods for studying desensitisation of cellular responses have been published. In this article, three commonly utilised protocols for studying homologous desensitisation of cellular responses are evaluated. These are (1) observation of attenuation of a response after an initial stimulus-induced activation, (2) repeated stimulation of cells after washing away the previous stimulus, and (3) repeated stimulation without a wash step. Advantages and limitations of each protocol are discussed and data is presented demonstrating some of the properties of the protocols.Copyright 1998 The Italian Pharmacological Society.

Language: English

Document Type: Research article

Affiliations: 1: Division of Hematology and Oncology, University of Michigan Medical Center, Ann Arbor, MI, USA 2: Departments of Surgery and Biological Chemistry, University of Michigan Medical Center and VA Medical Center, Research Service 11R, 2215 Fuller Road, Ann Arbor, MI, 48105, USA

Publication date: 1998-07-01