Effect of Tetra-Peptide Isolated from Interleukin 1 (IL-1) on Corneal Epithelial Wound Healing in the Rabbit
Source: Experimental Eye Research, Volume 72, Number 2, February 2001 , pp. 107-113(7)
Publisher: Academic Press
Abstract:To develop a new method for wound healing in case of injured corneal epithelium, the effects of the tetrapeptide (Val–Leu–Leu–Lys), showing the consensus sequence between human Interleukin (IL)-1α and bovine parotid protein (parotin) on epithelial cell proliferation and elongation were analysed in vitro cell culture experiments on epithelial cells obtained from rabbit cornea. The peptide showed dose-dependent stimulatory effects on epithelial cell proliferation and elongation at 10–100 g ml-1compared with the control experiments. Furthermore, the peptide also exhibited a significant wound healing activity for the epithelial cells in an in situ experiment using mechanically injured rabbit cornea, while the higher concentration of the peptide (100 g ml-1) showed greater efficient results than a previously known agent, sodium hyaluonate (0.3%). In addition, no pyrogenic activity of this peptide was detected by the previously established progenecity test using rabbits.
These results suggest that the tetrapeptide (Val–Leu–Leu–Lys) is a promising agent for wound healing in the case of injured corneal epithelium.
Document Type: Research Article
Affiliations: 1: Department of Ophthalmology, Nara Medical University, Nara, Kashihara-Shi, 630-8522, Japan 2: Department of Parasitology, Nara Medical University, Nara, Kashihara-Shi, 630-8522, Japan
Publication date: February 2001