Concentrative Nucleoside Transporter (rCNT1) Is Targeted to the Apical Membrane through the Hepatic Transcytotic Pathway

Authors: Duflot S.¹; Calvo M.²; Casado F.J.¹; Enrich C.²; Pastor-Anglada M.¹

Source: Experimental Cell Research, Volume 281, Number 1, November 2002 , pp. 77-85(9)

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Abstract:

The Na⁺-dependent nucleoside transporter CNT1 has been identified in a caveolin-enriched plasma membrane fraction (CEF), in transcytotic endosomes, and in canalicular membranes isolated from quiescent rat liver in which the transporter appears to be biologically active. CNT1 was also detected, albeit in small amounts, in the early/sorting endosomes. Plasma membrane preparations enriched in basolateral markers showed Na⁺-dependent nucleoside transport activity that is mostly, if not exclusively, accounted for by CNT2, a transporter protein which was not detected in CEF nor in the endosomal fractions. These data are consistent with different localization and trafficking pathways of the two isoforms in hepatocytes. CNT1 is the first transporter which is reported to follow the transcytotic pathway to be inserted on the apical side of liver parenchymal cells. © 2002 Elsevier Science (USA).

Keywords: concentrative nucleoside transporter (CNT); hepatocyte; endosome; endocytosis; transcytosis; caveolin

Language: English

Document Type: Research article

DOI: http://dx.doi.org/10.1006/excr.2002.5641

Affiliations: 1: Facultat de Biologia 2: Facultat de Biologia, Facultat de Medicina, Universitat de Barcelona, Barcelona, E-08028, Spain

Publication date: 2002-11-01