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Cryopreservation of Rhesus Macaque (Macaca mulatta) Spermatozoa and Their Functional Assessment by in Vitro Fertilization

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Although spermatozoa from several species of nonhuman primates have been cryopreserved, there has been no report of success with rhesus macaque spermatozoa as judged by functional assays. Two Tris–egg yolk freezing media, TEST and TTE, which have been successfully used for cynomolgus macaque (Macaca fascicularis) spermatozoa, were compared for cryopreservation of spermatozoa from four rhesus macaques (Macaca mulatta). The postthaw motility (percentage and duration) of spermatozoa cryopreserved in TTE was much higher than that for spermatozoa cryopreserved in TEST. The function of sperm cryopreserved in TTE was evaluated by in vitro fertilization of oocytes collected from gonadotropin-stimulated prepubertal rhesus macaques. Of the inseminated oocytes, 82 ± 13% were fertilized and 63 ± 22 and 39 ± 21% of the resulting zygotes developed into morulae and blastocysts, respectively. These results indicate that rhesus macaque spermatozoa can be effectively cryopreserved in TTE medium. This finding will facilitate the application of in vivo and in vitro assisted reproductive technologies in this species.

Keywords: Macaca mulatta; capacitation; cryopreservation; cryoprotectants; embryo development; glycerol; in vitro fertilization; plasma membrane; semen; sugar

Document Type: Research Article

Affiliations: 1: Kunming Institute of Zoology, The Chinese Academy of Science, Kunming, Yunnan, 650223, People's Republic of China 2: The China–U.S. Primate Biology Laboratory, Kunming Institute of Zoology and University of Wisconsin-Madison

Publication date: November 1, 2000

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