The Affinity of Binding of Digoxin to Ovine Anti-digoxin Fab (DIGIBIND )* Preparations

Author: Allen G.

Source: Biologicals, Volume 24, Number 1, March 1996 , pp. 19-24(6)

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Abstract:

Abstract. A fundamental study was performed to assess the binding characteristics of an ovine anti-digoxin-Fab preparation, DIGIBIND , for digoxin, using equilibrium dialysis. The average mass of the antibody fragment was determined by matrix-assisted laser-desorption mass spectrometry to be 46 200 Da. The main conclusions are that the binding affinity and capacity are similar for several batches, produced over a period of several years. The average median affinity for digoxin of five batches determined by Sips analysis was 3.4 x 10 M (range 1.95-6.13 x 10 M ) (corresponding to dissociation constants, Kd, in the range 1.63-5.13 p M ), and the index of heterogeneity was 0.78 ? 0.03.

Language: English

Document Type: Research article

Affiliations: Biology Division, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent, BR3 3BS, U.K.

Publication date: 1996-03-01