Inhibitory effect of endophyte bacteria on Botrytis cinerea and its influence to promote the grapevine growth

Authors: Ait Barka E.¹; Gognies S.²; Nowak J.³; Audran J-C.¹; Belarbi A.²

Source: Biological Control, Volume 24, Number 2, June 2002 , pp. 135-142(8)

Publisher: Academic Press

Abstract:

A grapevine pathogen, Botrytis cinerea, produces characteristic gray mold symptoms on leaves and/or stems within 7 days following the inoculation. In this study we used a plant growth-promoting rhizobacterium, Pseudomonas sp. strain PsJN, which demonstrates an induction of plant growth in parallel with an antagonistic effect on in vitro growth and development of B. cinerea. The simultaneous in vitro co-culture of B. cinerea and bacterium did not stop the spread of fungus. However, when the fungus was introduced 2 days after bacterium inoculation, a clear inhibition of B. cinerea appeared around the zone of Pseudomonas inoculation. When in vitro bacterized plantlets were challenged with B. cinerea, the symptoms of gray mold failed to develop compared to nonbacterized controls. Microscopic observation of B. cinerea mycelium from the zone of contact between the fungus and withPseudomonas sp. on the potato dextrose agar plate shows a growth disruption of fungal mycelium, coagulation, and leakage of protoplasm. The antagonist had no effect on the polygalacturonase activity of B. cinerea, meaning that the bacterium is not acting directly on the polygalacturonase of the fungus. It seems that the PsJN inhibits the growth ofB. cinerea by disrupting cellular membranes and inducing cell death.

© 2002 Elsevier Science (USA)

Language: English

Document Type: Research article

Affiliations: 1: Universite´ de Reims, UFR Sciences, B.P. 1039, URVVC, UPRES EA 2069, Laboratoire de Biologie et Physiologie Ve´ge´tales, 51687, Reims Cedex 2, France 2: Universite´ de Reims, UFR Sciences, B.P. 1039, URVVC, UPRES EA 2069, Laboratoire de Microbiologie Ge´ne´rale et Mole´culaire, 51687, Reims Cedex 2, France 3: Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA

Publication date: 2002-06-01

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• In this Subject: Plant Culture
• By this author: Ait Barka E. ;  Gognies S. ;  Nowak J. ;  Audran J-C. ;  Belarbi A.

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