TGF-1 Inhibits Protracted-relapsing Experimental Autoimmune Encephalomyelitis by Activating Dendritic Cells

Authors: Jin Y-X.; Xu L-Y.; Guo H.; Ishikawa M.; Link H.; Xiao B-G.

Source: Journal of Autoimmunity, Volume 14, Number 3, May 2000 , pp. 213-220(8)

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Abstract:

Protracted-relapsing experimental autoimmune encephalomyelitis (PR-EAE) in DA rats is an animal model closely related to multiple sclerosis (MS). Previous studies showed that nasal administration of TGF- beta 1 suppressed the development and relapse of PR-EAE clinically and pathologically. Here we demonstrate that this suppressive effect was associated with activation of dendritic cells (DC), showing elevated proliferative response and IFN- gamma and nitric oxide (NO) production by DC. DC derived from TGF- beta 1-treated rats with PR-EAE also showed high expression of nitric oxide synthase 2 (NOS2) at both mRNA and protein levels. Apoptotic cells were increased in spleen sections of TGF- beta 1-treated rats compared to control rats. In studying mechanisms of apoptosis in TGF- beta 1-treated rats, in vitro experiments demonstrated that TGF- beta 1-treated DC induced apoptosis of CD4⁺T cells by a NO pathway after co-culture with T cells. These results support the hypothesis that TGF- beta 1-induced suppression of PR-EAE is associated with apoptosis of CD4⁺T cells induced by DC-derived NO. Copyright 2000 Academic Press

Language: English

Document Type: Research article

Affiliations: Division of Neurology, Units of Experimental Neurology and Neuroimmunology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden

Publication date: 2000-05-01