TGF-
1 Inhibits Protracted-relapsing Experimental Autoimmune Encephalomyelitis by Activating Dendritic Cells
Authors: Jin Y-X.; Xu L-Y.; Guo H.; Ishikawa M.; Link H.; Xiao B-G.
Source: Journal of Autoimmunity, Volume 14, Number 3, May 2000 , pp. 213-220(8)
Publisher: Academic Press
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Abstract:
Protracted-relapsing experimental autoimmune encephalomyelitis (PR-EAE) in DA rats is an animal model closely related to multiple sclerosis (MS). Previous studies showed that nasal administration of TGF-1 suppressed the development and relapse of PR-EAE clinically and pathologically. Here we demonstrate that this suppressive effect was associated with activation of dendritic cells (DC), showing elevated proliferative response and IFN-
and nitric oxide (NO) production by DC. DC derived from TGF-1-treated rats with PR-EAE also showed high expression of nitric oxide synthase 2 (NOS2) at both mRNA and protein levels. Apoptotic cells were increased in spleen sections of TGF-1-treated rats compared to control rats. In studying mechanisms of apoptosis in TGF-1-treated rats, in vitro experiments demonstrated that TGF-1-treated DC induced apoptosis of CD4+T cells by a NO pathway after co-culture with T cells. These results support the hypothesis that TGF-1-induced suppression of PR-EAE is associated with apoptosis of CD4+T cells induced by DC-derived NO. Copyright 2000 Academic Press
Language: English
Document Type: Research article
Affiliations: Division of Neurology, Units of Experimental Neurology and Neuroimmunology, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden:
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