Equilibrium expert: an add-in to Microsoft Excel for multiple binding equilibrium simulations and parameter estimations

Authors: Raguin O.^{1, 2}; Gruaz-Guyon A.^{1, 2}; Barbet J.^{1, 3}

Source: Analytical Biochemistry, Volume 310, Number 1, November 2002 , pp. 1-14(14)

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Abstract:

An add-in to Microsoft Excel was developed to simulate multiple binding equilibriums. A partition function, readily written even when the equilibrium is complex, describes the experimental system. It involves the concentrations of the different free molecular species and of the different complexes present in the experiment. As a result, the software is not restricted to a series of predefined experimental setups but can handle a large variety of problems involving up to nine independent molecular species. Binding parameters are estimated by nonlinear least-square fitting of experimental measurements as supplied by the user. The fitting process allows user-defined weighting of the experimental data. The flexibility of the software and the way it may be used to describe common experimental situations and to deal with usual problems such as tracer reactivity or nonspecific binding is demonstrated by a few examples. The software is available free of charge upon request.

Language: English

Document Type: Research article

DOI: http://dx.doi.org/10.1016/S0003-2697(02)00289-0

Affiliations: 1: CNRS GDR 2352, Immunociblage des tumeurs, Marseille, France 2: INSERM U339, Hoˆpital Saint-Antoine, Paris, France 3: INSERM U463, Institut de Biologie, 9 quai Moncousu, 44093 Nantes Cedex 01, France

Publication date: 2002-11-01