IngentaConnect Flow Injection Spectrophotometric Determination of the Antibiotic...

Authors: Tzanavaras P.D.; Themelis D.G.

Source: Analytical Biochemistry, Volume 304, Number 2, May 2002 , pp. 244-248(5)

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Abstract:

A new flow injection (FI) method for the precise and rapid spectrophotometric determination of the antibiotic fosfomycin (FMC) in urine and pharmaceutical samples is described. The method is based on the on-line quantitative thermal-induced digestion of the analyte prior to injection into the FI system. Ammonium persulfate was used as the oxidation reagent. The resulting orthophosphate ions were determined spectrophotometrically ( lambda _max = 690 nm) using the molybdenum blue approach. Chemical and FI variables that affected on-line oxidation were studied and optimized. The proposed method is very precise (s_r = 1.2% at 1.0 × 10^-4 mol L^-1 FMC, n = 12), offers a high sampling rate of 60 h^-1, and allows for the determination of the analyte in the range 3.0 × 10^-6 to 3.0 × 10^-4 mol L^-1 with a satisfactory 3 sigma detection limit of 1.0 × 10^-6 mol L^-1. Application of the proposed method to urine and pharmaceutical samples yielded accurate results with percentage recoveries in the range 96.4–102.5%. © 2002 Elsevier Science (USA).

Keywords: fosfomycin; flow injection; on-line thermal-induced digestion; urine; pharmaceuticals; molybdenum blue

Language: English

Document Type: Research article

Affiliations: Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, 54006, Greece:

Flow Injection Spectrophotometric Determination of the Antibiotic Fosfomycin in Pharmaceutical Products and Urine Samples after On-line Thermal-Induced Digestion