Reporter Genes lucFF, luxCDABE, gfp, and dsred Have Different Characteristics in Whole-Cell Bacterial Sensors

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In this Subject: <a title="Anatomy & Physiology" href="/content/subcat?j_subject=157&j_availability=">Anatomy & Physiology ,&nbsp; Biology ,&nbsp; Organic Chemistry ,&nbsp; Biochemistry
By this author: Hakkila K. ;&nbsp; Maksimow M. ;&nbsp; Karp M. ;&nbsp; Virta M.

Authors: Hakkila K.; Maksimow M.; Karp M.; Virta M.

Source: Analytical Biochemistry, Volume 301, Number 2, February 2002 , pp. 235-242(8)

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Abstract:

The selection of a genetic reporter can be difficult because of the wide range of genes available. In order to reduce the selection, we compared the performance of different reporter genes: firefly luciferase (Photinus pyralis lucFF), bacterial luciferase operon (Photorhabdus luminescens luxCDABE), green fluorescent protein (Aequorea victoria gfp), and red fluorescent protein (Discosoma sp. dsred) in whole-cell bacterial sensors. Escherichia coli sensor bacteria were engineered to contain a reporter plasmid that carries the reporter gene under the control of mercury- (mer from Tn21) or arsenite- (ars from R773) responsive regulatory units. Characteristics of the strains were studied by using different arsenite or mercury concentrations and incubation times. The lowest detectable concentration of analytes and the fastest responses were achieved with lucFF or luxCDABE as reporter genes. The fluorescent proteins, GFP and DsRed, gave responses at higher analyte concentrations and after significantly longer incubation times. The results indicate that luciferases are better reporters in whole-cell sensor bacteria. ©2002 Elsevier Science (USA).