Supercritical Fluid Extraction and High-Performance Liquid Chromatography-Diode Array-Electrochemical Detection of Signature Redox Compounds from Sand and Soil Samples

Authors: Lang Q.1; Cheng I.F.1; Wai C.M.1; Paszczynski A.2; Crawford R.L.2; Barnes B.3, 5; Anderson T.J.4, 5; Wells R.5; Corti G.4, 5; Allenbach L.2; Erwin D.P.2; Assefi T.5; Mojarradi M.6

Source: Analytical Biochemistry, Volume 301, Number 2, February 2002 , pp. 225-234(10)

Publisher: Academic Press

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Abstract:

A supercritical fluid extraction procedure and a chromatographic separation/detection method were developed for the detection of Earth-based microorganisms. After microbes in a sand or a soil sample were hydrolyzed in a diluted NH4OH/acetone solution, several redox compounds from bacteria could be effectively extracted with trimethylamine-modified supercritical CO2 at 35°C and 300 atm. These signature redox-active compounds were separated by a reversed-phase HPLC column in an ion-pair mode and then monitored with a diode array detector and an electrochemical detector. The analytical results demonstrated the feasibility of using the reported techniques to detect the chemical signature of life in barren desert sand samples. ©2002 Elsevier Science (USA).

Keywords: SFE; DAD; ECD; HPLC; redox; riboflavin; coenzyme Q; FMN; FAD; hemin

Language: English

Document Type: Research article

Affiliations: 1: Department of Chemistry 2: Department of Chemistry, Environmental Biotechnology Institute 3: Department of Chemistry, Environmental Biotechnology Institute, Department of Electrical Engineering 4: Department of Chemistry, Environmental Biotechnology Institute, Department of Electrical Engineering, Department of Mechanical Engineering 5: Department of Chemistry, Environmental Biotechnology Institute, Department of Electrical Engineering, Department of Mechanical Engineering, Microelectronics Research and Communication Institute, University of Idaho, Moscow, Idaho, 83844 6: Jet Propulsion Laboratory, California Institute of Technology, Pasadena, California, 91109

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