Bioelectrochemical Analysis of Neuropathy Target Esterase Activity in Blood
Source: Analytical Biochemistry, Volume 290, Number 1, March 2001 , pp. 1-9(9)
Publisher: Academic Press
Abstract:Bioelectrochemical analysis of neuropathy target esterase (NTE) and its inhibitors is based on the combination of the NTE-catalyzed hydrolysis of phenyl valerate and phenol detection by a tyrosinase carbon-paste electrode. The use of the tyrosinase electrode improves 10-fold the sensitivity of NTE detection in comparison with a spectrophotometric method. The tyrosinase electrode was found to be suitable for measurements in whole human blood where spectrophotometric detection is considerably restricted. The specificity of NTE in blood for mipafox and di-2-propyl phosphorofluoridate was close to that for neuronal NTE. The NTE-like activity in blood was determined to be 0.19 ± 0.02 nmol/min/mg of protein.
Document Type: Research Article
Affiliations: 1: Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, 119899, Russia 2: Department of Analytical Biochemistry, Potsdam University, Im Biotechnologiepark, Luckenwalde, 14943, Germany 3: Research Center for Molecular Diagnostics and Therapy, Moscow, 113149, Russia 4: Russian Academy of Sciences, Chernogolovka, 142432, Russia
Publication date: March 1, 2001