Spectrofluorometric Determination of Putrescine Using Orthophthaldehyde as a Fluorophore: A Novel Method
This paper concerns spectrofluorometric analysis of putrescine using orthophthaladehyde as a fluorophore in aqueous alkaline medium. Wavelengths of excitation and emission in acid, neutral, and alkaline media were different. There is a maximum intensity of fluorescence in alkaline medium
24 h after complexation compared with other media. Putrescine and orthophthaladehyde are used at an equimolar ratio, and the product is kinetically stable in alkaline medium. Calibration curves obtained gave limits of detection and quantification of 39 and 65 ng/mL, respectively. The correlation
coefficient obtained in alkaline medium was 0.992 at pH 12. Results obtained largely showed a good reproducibility of our method.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics
Document Type: Research Article
Affiliations: 1: University of The Gambia, School of Arts and Sciences, Division of Physical and Natural Sciences, Gambia 2: Université Cheikh Anta Diop, Faculté des Sciences et Techniques, Département de Chimie, Laboratoire de Photochimie et d’Analyse, Dakar, Sénégal 3: Université Littorale Côte d’Opale, Unité de Chimie Environnementale et Interactions sur le vivant, B.P. 59140, Dunkerque, France
Publication date: 01 February 2016
This article was made available online on 05 February 2016 as a Fast Track article with title: "Spectrofluorometric Determination of Putrescine Using Orthophthaladehyde as a Fluorophore: A Novel Method".
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Journal Information
- Masthead
- Ingenta Connect is not responsible for the content or availability of external websites
Receive new issue alert