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Surface Plasmon Resonance Biosensor for Determination of Tetrodotoxin: Prevalidation Study

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Abstract:

A label-free surface plasmon resonance biosensor method was applied to determine tetrodotoxin (TTX) in pufferfish matrixes using an antibody inhibition assay format. A prevalidation study was conducted to demonstrate the assay performance characteristics, such as selectivity, LOD, LOQ, repeatability, reproducibility, and accuracy. Three participating laboratories reported standard curves in buffer and pufferfish matrix. A set of blind samples with TTX spiked into buffer as well as in 10 pufferfish extract were analyzed. Additionally, three blind naturally contaminated samples were analyzed, and the results were compared to those obtained using a reference method (HPLC/electrospray ionization-selected reaction monitoring-MS). The developed method was demonstrated to be capable of detecting TTX in pufferfish matrix standard samples in a broad concentration range (29000 ng/mL) with an LOD of 1.5 ng/mL. Between-laboratory recovery values were in the range of 51190 with a mean of 107, and 64180 with a mean of 103 for TTX-spiked samples in buffer and pufferfish matrix, respectively. Between-laboratory recoveries were in the satisfactory range of 101119 for naturally contaminated samples. This robust, rapid, and noninvasive method may serve as an attractive alternative to established methods for detection of TTX in pufferfish extracts.

Document Type: Research Article

Affiliations: 1: University of Washington, Department of Chemical Engineering, Box 351750, Seattle, WA 98195 and Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chabersk 57, 182 51 Prague, Czech Republic. 2: University of Washington, Department of Chemical Engineering, Box 351750, Seattle, WA 98195. 3: Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chabersk 57, 182 51 Prague, Czech Republic. 4: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD 20740.

Publication date: March 1, 2011

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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