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Simultaneous Determination of Seven Alkaloids in Phellodendron chinense Schneid by High-Performance Liquid Chromatography

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By optimizing the extraction, separation, and analytical conditions, a reliable and accurate HPLC method coupled with a photodiode array detector was developed for simultaneous detection and quantification of seven alkaloids, i.e., (-)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, chilenine, and pteleine in huangbo (the bark of Phellodendron chinense), a commonly used herb in Traditional Chinese Medicine. The optimal condition for separation was achieved on a reversed-phase C18 column with a stepwise mobile phase gradient prepared from 0.1 phosphoric acid and acetonitrile. For all the alkaloids, a good linear regression relationship (r > 0.9997) was obtained between the peak area and concentration at the range of 0.5700 g/mL. The LODs and LOQs for the analytes ranged from 0.07 to 0.28 g/mL and from 0.28 to 1.12 g/mL, respectively. The optimized method was applied to the determination of alkaloids in several P. chinense samples, and found to be feasible and reliable. This method and quantitative results can provide scientific and technical bases for setting up QC standards to assure the safety and quality of P. chinense bark raw material, as well as for proprietary Chinese medicine products containing P. chinense bark.
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Document Type: Research Article

Affiliations: 1: Chinese Academy of Sciences, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Lanzhou 730000, People's Republic of China and Graduate University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China. 2: Chinese Academy of Sciences, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Lanzhou 730000, People's Republic of China.

Publication date: 01 September 2010

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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