Determination of Ethoxyquin by High-Performance Liquid Chromatography with Fluorescence Detection and Its Application to the Survey of Residues in Food Products of Animal Origin

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Abstract:

An analytical method using HPLC with fluorescence detection (HPLC-FL) has been developed and applied for the survey of residue levels of ethoxyquin in a variety of food products of animal origin. HPLC was performed using a silica octadecylsilane column, butylhydroxytolueneacetonitrilewater (0.05 + 800 + 200, v/v/v) mobile phase, and detection at excitation and emission wavelengths of 370 and 415 nm, respectively. HPLC/MS was used to confirm whether a chromatographic peak was ethoxyquin. The LOQ of the foods was 0.01 g/g, except for pig fat and cow's milk, and the RSD (n = 6) at 0.1 g/mL of the standard solution was 1.12. The accuracy of the calculated data of the standard solution was within the range of 94.0 to 101.2. Recoveries of ethoxyquin from the food products of cattle, pigs, chickens, and salmon were more than 71.0 with an RSD of <9.3, except for chicken liver at different concentration levels, including the lower LOQ, the maximum residue limit (MRL), and in some tissues, twice the MRL. Residue levels of ethoxyquin in 33 commercially available food products of animal origin that were purchased on the west side of the Tokyo metropolitan area were surveyed. Contents of ethoxyquin residues in three chicken fat samples by the HPLC-FL method were 0.08, 0.03, and 0.04 g/g, all less than the MRL (5 g/g).

Document Type: Research Article

Affiliations: 1: Research Institute for Animal Science in Biochemistry and Toxicology, Department of Analytical Chemistry, 3-7-11 Hashimotodai, Sagamihara, Kanagawa 229-1132 Japan. 2: Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Department of Analytical Chemistry, 1432-1 Horinouchi, Hachioji 192-0392 Tokyo, Japan.

Publication date: January 1, 2010

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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