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Development and Single-Laboratory Validation of a Method for the Determination of Total Fructans in Infant Formula

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A number of methods have previously been developed for the determination of fructans in food products, resulting in the frequently used AOAC Official Methods SM 997.08 and 999.03. Method 997.08 has a lower LOQ, but the accuracy may suffer if significant quantities of sucrose are present. Method 999.03 is less affected by sucrose, but the LOQ is not as low as that of the other method. In this work we have combined these two methods, using the sample preparation steps of AOAC Method 999.03 to remove sucrose and other free sugars, and the detection procedure of AOAC Method 997.08 to achieve a low LOQ. The resulting new method achieved an LOQ of 0.13 g/100 g with powdered products (using 1 g sample weight) and recoveries in the range of 83103 in the presence of sucrose at 12 g/100 g. Expanded measurement uncertainties were calculated and ranged from 17.4 at oligofructose and fructooligosaccharide (FOS) concentrations of around 0.2 g/100 g to 21.0 at FOS concentrations of 0.6 g/100 g.
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Document Type: Research Article

Affiliations: Nestl Research Centre, Vers-Chez-Les-Blanc, PO Box 44, CH-1000 Lausanne 26, Switzerland.

Publication date: 2010-01-01

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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