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Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry

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Abstract:

A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2 (v/v) formic acid in methanolacetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.580.0 ng/mL with r 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.

Document Type: Research Article

Affiliations: 1: Torrent Research Center, Bioanalytical Laboratory, Gandhinagar382428, Gujarat State, India and Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat State, India. 2: Torrent Research Center, Bioanalytical Laboratory, Gandhinagar382428, Gujarat State, India. 3: Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat State, India.

Publication date: 2010-01-01

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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