Determination of Metformin Hydrochloride and Glyburide in an Antihyperglycemic Binary Mixture Using High-Performance Liquid Chromatographic-UV and Spectrometric Methods
Abstract:Three methods were developed for simultaneous determination of metformin hydrochloride and glyburide in an antihyperglycemic binary mixture without previous separation. In the first method, a reversed-phase HPLC column with acetonitrilewater (60 + 40, v/v) mobile phase at 0.9 mL/min flow rate was used to separate both compounds, with UV detection at 254 nm. Linearity was obtained in the concentration range of 0.060.24 g/mL for glyburide and 1.56.0 g/mL for metformin hydrochloride. The second method depended on first- and second-derivative UV spectrometry with zero-crossing measurements. The first-derivative amplitude at 261 nm was selected for the assay of glyburide, and the second-derivative amplitude at 235 nm was selected for the assay of metformin hydrochloride. The third method depended on measuring the first derivative of the ratio-spectra at 241 nm for glyburide and 227 nm for metformin hydrochloride. For the second and third methods, Beer's law was obeyed in the range of 1055 g/mL for glyburide and 20200 g/mL for metformin. The proposed methods were extensively validated and applied for the analysis of some pharmaceutical formulations containing binary mixtures of the mentioned drugs.
Document Type: Research Article
Affiliations: Minia University, Faculty of Pharmacy, Analytical Chemistry Department, Minia, Egypt.
Publication date: January 1, 2010
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