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Quantitative Analysis of the Neonicotinoid Insecticides Imidacloprid and Thiamethoxam in Fruit Juices by Enzyme-Linked Immunosorbent Assays

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Rapid and sensitive ELISAs have been applied to analyze imidacloprid and thiamethoxam residues in apple, grape, orange, and peach juices after simple dilution of the samples without any extraction and cleanup. The matrix interference of the fruit juices was eliminated after 100-fold dilution, and the ELISAs gave method LODs of imidacloprid and thiamethoxam in fruit juices down to 20 and 5 ng/g, respectively. Average recoveries of imidacloprid and thiamethoxam from the spiked fruit juices were in the range of 92115 and 87118, respectively. The CVs of the recoveries ranged from 3 to 19 and from 6 to 19 for imidacloprid and thiamethoxam, respectively. The ELISA results were comparable to those obtained by a reference HPLC/MS method using pesticide-spiked samples, with reasonable correlation coefficients between 0.89 and 0.93 and regression coefficients (slopes) between 0.75 and 0.94.
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Document Type: Research Article

Affiliations: 1: China Agricultural University, College of Resources and Environmental Sciences, Beijing 100193, People's Republic of China. 2: China Agricultural University, Department of Applied Chemistry, Beijing 100193, People's Republic of China. 3: University of Hawaii, Department of Molecular Biosciences and Bioengineering, Honolulu, HI 96822.

Publication date: 2010-01-01

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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