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Evaluation of Immunoassays as an Alternative for the Rapid Determination of Pesticides in Wine and Grape Samples

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Abstract:

The main objective of this paper is to address the performance of immunochemical assays for the detection of the residues of three pesticides [atrazine, bromopropylate, and 2,4,6-trichlorophenol (TCP)] in real winery samples, such as wine, grapes, and grape juice. Different approaches have been evaluated to minimize interferences from the matrixes, and suitable working protocols have been established in order to achieve the necessary LODs, accuracy, and precision for real samples. A simple dilution of the sample proved to be sufficient for the determination of atrazine and bromopropylate in red and white wine and grape juice at the required levels of concentration. However, for TCP, an SPE procedure has been optimized using amino cartridges. The recoveries were above 85 in all cases, and the LOD values were below the parts per billion level, except for bromopropylate, which ranged between 2 and 50 g/L, depending on the matrix. The grape matrix effect could be resolved by a simple extraction with methanol. Complete recoveries were obtained, and the final measurement procedures were able to determine selected pesticides below their maximum residue levels. The newly developed methods have been compared with standard chromatographic methods.

Document Type: Research Article

Affiliations: 1: AZTI-Tecnalia, Food Research Division, 48160 Derio, (Bizkaia) Spain. 2: Applied Molecular Receptors Group (AMRg), Advanced Chemical Research Institute (IQAC), Spanish Council for Scientific Research, Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), c/Jordi Girona 18-26, 08034 Barcelona, Spain. 3: Applied Molecular Receptors Group (AMRg), Advanced Chemical Research Institute (IQAC), Spanish Council for Scientific Research, Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), c/Jordi Girona 18-26, 08034 Barcelona, Spain.

Publication date: January 1, 2010

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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