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Determination of Erythromycin and Tylosin Residues in Honey by LC/MS/MS

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Abstract:

Antibiotics are used in apiculture to protect bees against a variety of brood diseases. As a result of the development of resistance to oxytetracycline, erythromycin and tylosin are increasingly used for the prevention and treatment of these diseases. Therefore, Brazilian authorities have added these antibiotics to the National Regulatory Monitoring Program for the control of residues in honey. An analytical method has been developed for the determination of residues of erythromycin and tylosin in honey. The procedure involves solid-phase extraction of diluted honey samples with Bond Elut cartridges, followed by LC/MS with electrospray positive ionization in the multiple reaction monitoring mode. Two characteristic transitions were monitored for both drugs. Average analyte recoveries of erythromycin and tylosin ranged from 99 to 109 from sets of replicate honey samples fortified with drug concentrations of 5, 10, 15, and 20 g/kg. The method decision limits were determined to be 1.27 and 0.59 g/kg for erythromycin and tylosin, respectively. The detection capabilities were 5 and 5.2 g/kg for erythromycin and tylosin, respectively.

Document Type: Research Article

Affiliations: 1: Microbioticos Laboratories, Av. Santa Isabel 2120, Campinas, SP, 13084-471, Brazil. 2: University of London, Centre for Chemical and Bioanalytical Sciences, School of Biological Sciences, Royal Holloway, Egham, Surrey TW20 OEX, United Kingdom.

Publication date: May 28, 2009

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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