Optimization of the Treatment of Wheat Samples for the Determination of Phytic Acid by HPLC with Refractive Index Detection

Authors: Amaro, Rosa; Murillo, Miguel; Gonzlez, Zurima; Escalona, Andrs; Hernndez, Lus

Source: Journal of AOAC International, Volume 92, Number 3, May 2009 , pp. 873-878(6)

Publisher: AOAC International

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Abstract:

The treatment of wheat samples was optimized before the determination of phytic acid by high-performance liquid chromatography with refractive index detection. Drying by lyophilization and oven drying were studied; drying by lyophilization gave better results, confirming that this step is critical in preventing significant loss of analyte. In the extraction step, washing of the residue and collection of this water before retention of the phytates in the NH2 Sep-Pak cartridge were important. The retention of phytates in the NH2 Sep-Pak cartridge and elimination of the HCl did not produce significant loss (P = 0.05) in the phytic acid content of the sample. Recoveries of phytic acid averaged 91, which is a substantial improvement with respect to values reported by others using this methodology.

Document Type: Research Article

Affiliations: Universidad Central de Venezuela, Facultad de Ciencias, Escuela de Qumica, Centro de Qumica Analtica, Laboratorio de Mtodos Cromatogrficos, Apartado 47102, Caracas 1041-A, Venezuela.

Publication date: May 28, 2009

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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