Separation and Determination of Clenbuterol by HPLC Using a Vancomycin Chiral Stationary Phase
Enantiomers of clenbuterol were separated by a new HPLC method on a chiral column. Enantiomeric resolution was achieved on a vancomycyin macrocyclic antibiotic chiral stationary phase known as chirobiotic V with UV detection at 247 nm. The polar ionic mobile phase consisting of methanoltriethylamineglacial acetic acid (100 + 0.05 + 0.025, v/v/v), was used at a flow rate of 1.0 mL/min. The method was validated for linearity, accuracy, precision, and robustness. Standard linear calibration curves were established for the R-() and S-(+) enantiomers over the range of 0.220 g/mL, and an average recovery of 98.0 and a mean relative standard deviation of 1.5 were obtained at 5.0 g/mL. The lower limit of detection was 0.05 g/mL for each enantiomer. The mean recovery for R-() and S-(+)-clenbuterol enantiomers from plasma was 91.097.0 at 0.2020 g/mL. The method was successfully used to identify and quantify the clenbuterol enantiomers in human plasma.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
Document Type: Research Article
Affiliations: King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, PO Box 2457, Riyadh 11451, Saudi Arabia.
Publication date: 2009-05-28
More about this publication?
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
- Information for Authors
- Submit a Paper
- Subscribe to this Title
- Information for Advertisers
- Journal Information
- Ingenta Connect is not responsible for the content or availability of external websites