If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email firstname.lastname@example.org
Many polyherbal oil formulations in Indian and Chinese traditional systems of medicine used for control of skin diseases contain seeds of Psoralea corylifolia and roots of Plumbago zeylanica L. Psoralen and plumbagin are the reliable markers for Ps. corylifolia
and Pl. zeylanica, respectively. However, no attempt is made to standardize the polyherbal oil formulations containing Ps. corylifolia and Pl. zeylanica in terms of their active ingredients or marker compounds. In this paper, a simple, rapid, and sensitive HPTLC method
is described for the first time to identify and quantify psoralen and plumbagin from such polyherbal oil formulations. The methanolic extract of oil formulations was used for analysis of markers. Psoralen gives a sharp UV absorbance peak at 302 nm and plumbagin at 275 nm. Good resolution of
psoralen (Rf = 0.37) and plumbagin (Rf = 0.77) was attained using tolueneethyl acetate (7.5 + 2.5, v/v) mobile phase. The method was validated in terms of calibration curve, limits of detection and quantification, precision, accuracy, and robustness following a standard
protocol. Polyherbal oil formulations were analyzed with reasonable accuracy, and no matrix interference was observed. The method developed can be used for marker-based quality assurance of oil formulations containing Ps. corylifolia and Pl. zeylanica as one active ingredient.
Document Type: Research Article
Devi Ahilya Vishwa Vidyalaya, School of Pharmacy, Indore, India. 2:
Indore Professional Studies Academy, College of Pharmacy, Indore, India. 3:
Vallabh Vidyanagar, A.R. College of Pharmacy, Gujarat, India.
Publication date: May 28, 2009
More about this publication?
The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.