Skip to main content

Determination of Chloramphenicol Residues in Bee Pollen by Liquid Chromatography/TandemMass Spectrometry

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

A novel liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method was developed for the trace residue determination of chloramphenicol (CAP) in bee pollen. CAP was extracted from bee pollen with a mixture of methanol and 1 metaphosphoric acid solution, followed by a 2-stage solid-phase extraction enrichment and cleanup. The first stage involved a polymeric cartridge, and the second stage involved an alumina neutral cartridge. The LC separation was performed on a C18 column with 10 mM ammonium formateacetonitrile (7 + 3) as the mobile phase and MS detection with negative-ion electrospray ionization. CAP- d5 was used as the internal standard. The method was validated according to Commission Decision 2002/657/EC. The calibration curves were linear between 0.1 and 5.0 ng/mL, and overall recoveries ranged from 98 to 113. Decision limits (CC) ranged from 0.05 to 0.07 g/kg, and detection capabilities (CC) ranged from 0.08 to 0.12 g/kg. The developed method was applied to 11 samples.

Document Type: Research Article

Affiliations: 1: Japan Food Research Laboratories, 7-4-41, Saitoasagi, Ibaraki-shi, Osaka 567-0085, Japan. 2: Mimasaka University, 50 Kitazono-cho, Tsuyama, Okayama 708-8511, Japan.

Publication date: September 1, 2008

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Journal Information
  • Masthead
  • ingentaconnect is not responsible for the content or availability of external websites
aoac/jaoac/2008/00000091/00000005/art00014
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more