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Sensitive and Rapid Method to Determine Trimetazidine in Human Plasma by Liquid Chromatography/Tandem Mass Spectrometry

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Abstract:

A simple, sensitive, and rapid liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method, using electrospray ionization, was developed and validated to quantify trimetazidine in human plasma using propranolol hydrochloride as an internal standard (IS). Samples were prepared by solid-phase extraction and analyzed without drying and reconstitution. The analyte and IS were chromatographed on a C18 reversed-phase column under isocratic conditions using 2 mM ammonium acetate (pH 3.5) acetonitrile (40 + 60, v/v) as the mobile phase with a run time of 2.0 min. Quantitation was done on a triple-quadrupole mass analyzer API-3000, equipped with turbo ion spray interface and operating in multiple reaction monitoring mode to detect parent product ion (m/z 267.2 181.4) transition. The method was validated for sensitivity, accuracy and precision, linearity, recovery, matrix effect, and stability. Linearity in plasma was observed over the concentration range of 1.5300 ng/mL. Lower limit of quantification achieved was 1.5 ng/mL with precision <10 using 10 L injection volume. The mean relative recovery of analyte (97.36) and IS (99.93) was consistent and reproducible. Interbatch and intrabatch precision was <8.0 and the accuracy determined was within 8 in terms of relative error.

Document Type: Research Article

Affiliations: 1: Gujarat University, Department of Chemistry, School of Sciences, Navrangpura, Ahmedabad 380 009, India; Accutest Research Lab, Satellite, Ahmedabad 380015, India. 2: Accutest Research Lab, Satellite, Ahmedabad 380015, India. 3: Gujarat University, Department of Chemistry, School of Sciences, Navrangpura, Ahmedabad 380 009, India.

Publication date: May 1, 2008

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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