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Development and Validation of an HPLC Method to Quantify Camptothecin in Polymeric Nanocapsule Suspensions

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A simple, rapid, and sensitive reversed-phase column high-performance liquid chromatographic method was developed and validated to quantify camptothecin (CPT) in polymeric nanocapsule suspensions. The chromatographic separation was performed on a Supelcosil LC-18 column (15 cm 4.6 mm id, 5 m) using a mobile phase consisting of methanol10 mM KH2PO4 (60 + 40, v/v; pH 2.8) at a flow rate of 1.0 mL/min and ultraviolet detection at 254 nm. The calibration graph was linear from 0.5 to 3.0 g/mL with a correlation coefficient of 0.9979, and the limit of quantitation was 0.35 g/mL. The assay recovery ranged from 97.3 to 105.0. The intraday and interday relative standard deviation values were <5.0. The validation results confirmed that the developed method is specific, linear, accurate, and precise for its intended use. The current method was successfully applied to the evaluation of CPT entrapment efficiency and drug content in polymeric nanocapsule suspensions during the early stage of formulation development.
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Document Type: Research Article

Affiliations: 1: Universidade Federal de Santa Catarina, Departamento de Cincias Farmacuticas, Laboratrio de Farmacotcnica, Campus Universitrio Trindade, Florianpolis, SC 88040-900, Brazil. 2: Universidade Federal de Santa Catarina, Departamento de Cincias Farmacuticas, Laboratrio de Controle de Qualidade, Campus Universitrio Trindade, Florianpolis, SC 88040-900, Brazil.

Publication date: 2008-05-01

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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