Single-Laboratory Validation of a Method for the Determination of c-Phycocyanin and Allophycocyanin in Spirulina (Arthrospira) Supplements and Raw Materials by Spectrophotometry

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

A single-laboratory validation study was conducted for a 2-wavelength spectrophotometric method for the determination of c-phycocyanin (cPC) and allophycocyanin (aPC) in Spirulina supplements and raw materials. The absorption maxima of cPC and aPC are at 620 and 650 nm, respectively. The concentrations of the analytes were calculated from measurement of absorbance at 2 wavelengths. This method provided linear responses for cPC and aPC in the range of 25250 g/mL. Duplicate determinations of cPC and aPC in 4 different test materials on 5 different days resulted in relative standard deviations of 0.31.0 and 1.01.5 for cPC and aPC, respectively. Recoveries were 99.2102.4 and 100.0104.0 for cPC and aPC, respectively. The results were satisfactory for the determination of cPC and aPC in Spirulina supplements.

Document Type: Research Article

Affiliations: 1: DIC LIFETEC Co., Ltd, Technical Department 12, Yawata-kaigandori, Ichihara, Chiba 290-8585, Japan. 2: Earthrise Nutritionals LLC, Technical Division, 113 E. Hoober Rd, Calipatria, CA 92233.

Publication date: May 1, 2008

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Journal Information
  • Masthead
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more