Determination of Phytase Activity in Feed: Interlaboratory Study
Abstract:An interlaboratory study was conducted to determine the performance characteristics of a new method for the determination of phytase activity in feed samples. The method is based on the principle that inorganic phosphate is released from the substrate phytate under defined assay conditions and has been validated for its suitability to measure the enzyme activity of various phytase products. Two different experimental designs of the study were applied, allowing for the estimation of the precision of the method under repeatability, intermediate precision and reproducibility conditions, respectively. The relative standard deviation for repeatability (RSDr) ranged from 2.2 to 10.6 and the RSD for reproducibility (RSDR) ranged from 5.4 to 15. The suitability of the validated method for the intended purpose was demonstrated. The obtained performance profile of the method validated in this study was comparable to that of similar methods that were exclusively validated for one phytase product.
Document Type: Research Article
Affiliations: 1: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, B-2440 Geel, Belgium. 2: FEFANA Asbl (European Union Feed Additives and Premixtures Association Manufacturers), Ave Louise 120, Box 13, B-1050 Brussels, Belgium; Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark. 3: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; Alltech EU Regulatory Dept, 14 Place Marie-Jeanne Bassot, 92300 Levallois-Perret, France. 4: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; DSM Nutritional Products Ltd, PO Box 3255, 4002 Basel, Switzerland. 5: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; Danisco A/S, Enzyme R&D, Edwin Rahrs Vej 38 DK-8220 Brabrand, Denmark. 6: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; BASF Aktiengesellschaft, GKA/O- E210, 67056 Ludwigshafen, Germany. 7: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; Alltech Bioscience Centre, Sarney, Summerhill Rd, Dunboyne, County Meath, Ireland. 8: FEFANA Asbl, Ave Louise 120, Box 13, B-1050 Brussels, Belgium; Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark.
Publication date: March 1, 2008
- The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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