Development and Validation of Liquid Chromatographic and Ultraviolet Derivative Spectrophotometric Methods for Determination of Epinastine Hydrochloride in Coated Tablets

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

High-performance liquid chromatographic (LC) and ultraviolet derivative spectrophotometric (UVD) methods were developed and validated for the quantitative determination of epinastine hydrochloride in coated tablets. LC was performed on a reversed-phase RP-18 column with a mobile phase composed of 0.3 triethylamine (pH adjusted to 4.0 with 10 orthophosphoric acid)methanol (60 + 40, v/v). The first-order derivative method was performed at 243.8 nm using HCl and methanol as the solvent. The methods were validated according to U.S. Pharmacopoeia and International Conference on Harmonization guidelines. The statistical analysis by Student's t-test showed no significant difference between the results obtained by the 2 methods. The proposed methods were found to be simple, rapid, precise, accurate, robust, and sensitive, allowing perfect interchange. The LC and UVD methods can be used in the routine quantitative determination of the epinastine hydrochloride in coated tablets.

Document Type: Research Article

Affiliations: 1: Universidade Federal do Rio Grande do Sul, Faculdade de Farmcia, Programa de Ps-Graduao em Cincias Farmacuticas, Porto Alegre, Brazil. 2: Universidade Federal do Rio Grande do Sul, Faculdade de Farmcia, Programa de Ps-Graduao em Cincias Farmacuticas, Porto Alegre, Brazil, and Universidade Federal do Rio Grande do Sul, Faculdade de Farmcia, Departamento de Produo, Controle de Qualidade de Medicamentos, Porto Alegre, Brazil. 3: Universidade Federal do Rio Grande do Sul, Faculdade de Farmcia, Programa de Ps-Graduao em Cincias Farmacuticas, Porto Alegre, Brazil, and Universidade Federal do Rio Grande do Sul, Faculdade de Farmcia, Departamento de Produo, Controle de Qualidade de Medicamentos, Porto Alegre, Brazil.

Publication date: September 1, 2007

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Information for Advertisers
  • Journal Information
  • Masthead
  • ingentaconnect is not responsible for the content or availability of external websites

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more